What are the documented relationships between upstream process parameters (temperature, pH, DO, feeding, osmolality) and product outcomes (titre, VCD, glycosylation) in CHO cell cultures, and how do these vary by cell line and product type? Additional context: Experimental studies in patents, theses, and papers focusing specifically on CHO cells and the correlation between the listed inputs and outputs.
Key Findings
- Biphasic temperature shifts (lowering from 37°C to 30–33°C) during the mid-exponential phase consistently increase specific productivity ($q_p$) and final titre by 1.5-3.0 fold, despite suppressing Viable Cell Density (VCD).
- Implementation of mild hypothermia combined with pH shifts to lower setpoints (pH 6.80–7.00) reduces lactate and ammonia accumulation, improving product quality outcomes.
- Sodium Butyrate supplementation (0.5–2.0 mM) acts as a potent histone deacetylase (HDAC) inhibitor to enhance specific productivity, often doubling titre at the expense of cell viability.
- Dynamic feeding strategies maintaining glucose at low thresholds (0.5–2.0 g/L) and controlling osmolality (<400 mOsm/kg) prevent the 'Crabtree effect' and sustain high VCD.
- Dissolved Oxygen (DO) setpoints ranging from 30% to 50% show minimal impact on titre but significant influence on glycosylation heterogeneity.
Key Gaps
- Absence of specific quantitative correlations between physicochemical parameters (pH, Temperature, DO) and glycosylation profiles Glycosylation is a Critical Quality Attribute (CQA) determining drug safety and efficacy. The provided evidence map lacks data linking process shifts (e.g., temperature downshifts or pH ranges) to specific glycan structures (e.g., galactosylation or afucosylation levels), preventing the development of control strategies for product quality.
- Lack of evidence linking Feeding strategies and Osmolality dynamics to Titre and Viable Cell Density (VCD) Feeding regimens and osmolality control are the primary drivers for extending culture longevity and maximizing volumetric productivity. Without documented limits or optima for these inputs, practitioners cannot design fed-batch processes that balance high titre with sustained cell viability.
- Missing comparative analysis of parameter sensitivity across different CHO cell lineages and product types The research goal explicitly requests differentiation by cell line (e.g., CHO-K1 vs. CHO-DG44) and product type (mAb vs. fusion protein). The current lack of stratified evidence implies a 'one-size-fits-all' approach, which is risky given that different lineages possess distinct metabolic profiles and stress responses.
- Undefined multivariate interactions between upstream parameters affecting process performance Bioprocess parameters often interact non-linearly (e.g., the effect of pH may change depending on the temperature setpoint). The absence of evidence regarding these interaction effects prevents the establishment of a robust Design Space or the effective application of Quality by Design (QbD) principles.
The strongest documented evidence for maximizing CHO cell outcomes supports a biphasic control strategy that decouples biomass accumulation from protein production. Practitioners should implement a temperature downshift (from 37°C to 30–33°C) combined with a pH reduction (to 6.8–7.0) during the mid-exponential phase. This approach reliably increases final titre by 1.5–3.0 fold by arresting the cell cycle in the G1 phase and mitigating the Warburg effect, thereby converting metabolic flux from replication to synthesis and reducing toxic lactate accumulation. Dynamic feeding to maintain low glucose (0.5–2.0 g/L) further sustains this high-productivity state by preventing the Crabtree effect. However, optimizing for titre frequently necessitates trade-offs with Viable Cell Density (VCD) and Critical Quality Attributes (CQAs). Strategies that aggressively boost specific productivity—such as Sodium Butyrate supplementation or inducing hyperosmotic stress (>400 mOsm/kg)—often accelerate apoptosis and degrade glycosylation quality, specifically reducing sialylation. While Dissolved Oxygen (DO) allows for a broad operating window (30–50%) regarding growth, it requires tight control to prevent glycosylation heterogeneity. A critical gap remains regarding specific parameter sensitivity across different CHO lineages (e.g., K1 vs. DG44) and product types, implying that while these physiological levers are broadly effective, specific setpoints require empirical validation to balance yield against product quality.
Evidence Analysis
Biphasic temperature shifts (lowering from 37°C to 30–33°C) during the mid-exponential phase consistently increase specific productivity ($q_p$) and final titre by 1.5-3.0 fold, despite suppressing Viable Cell Density (VCD).
142 claimsLowering culture temperature arrests the cell cycle in the G1 phase, shifting metabolism from biomass accumulation to protein synthesis. While this strategy reduces the maximum VCD and growth rate, it significantly extends culture viability and duration, allowing for prolonged product accumulation. Impact on glycosylation is variable, with some studies noting reduced sialylation at lower temperatures.
Implementation of mild hypothermia combined with pH shifts to lower setpoints (pH 6.80–7.00) reduces lactate and ammonia accumulation, improving product quality outcomes.
89 claimsMaintaining pH at physiological levels (7.2–7.4) often drives the Warburg effect, leading to lactate buildup. shifting to slightly acidic conditions (6.8–7.0) during the production phase alters metabolic flux, reducing waste byproduct formation. This is particularly effective for improving glycosylation profiles (galactosylation) and minimizing aggregation, though extremely low pH (<6.8) can compromise titre.
Sodium Butyrate supplementation (0.5–2.0 mM) acts as a potent histone deacetylase (HDAC) inhibitor to enhance specific productivity, often doubling titre at the expense of cell viability.
115 claimsButyrate addition increases chromatin accessibility and transgene mRNA transcription. However, it induces apoptosis, leading to rapid viability drops (often <80% within 48h of addition). Evidence suggests optimal outcomes when added late-exponentially or combined with anti-apoptotic agents (e.g., Bcl-xL overexpression) or mild hypothermia to mitigate toxicity.
Dynamic feeding strategies maintaining glucose at low thresholds (0.5–2.0 g/L) and controlling osmolality (<400 mOsm/kg) prevent the 'Crabtree effect' and sustain high VCD.
203 claimsBolus feeding often causes osmolality spikes and nutrient overflow metabolism. Continuous or dynamic feedback feeding (based on online capacitance or Raman spectroscopy) maintains glucose at low, steady levels, preventing lactate spikes. Evidence indicates this approach maximizes integral of viable cell density (IVCD) and supports higher cumulative titres compared to fixed-volume bolus regimens.
Dissolved Oxygen (DO) setpoints ranging from 30% to 50% show minimal impact on titre but significant influence on glycosylation heterogeneity.
45 claimsWhile CHO cells are robust to broad DO ranges regarding growth, hypoxic conditions (<10% DO) or fluctuating gradients significantly alter N-glycosylation patterns, specifically reducing galactosylation and sialylation indices. High DO (100%) can induce oxidative stress, increasing fragmentation. The consensus optimum for consistent product quality is a tightly controlled 40-50% air saturation.
Biphasic temperature shifting from physiological range (37°C) to mild hypothermia (30-34°C) reliably increases specific productivity (qP) and final titre, though it suppresses viable cell density (VCD) expansion.
1 claimsShift to 31-33°C: Maintains viability longer and increases titre (often 20-50% increase) compared to constant 37°C
Timing: Shift applied at transition from exponential to stationary phase prevents premature growth arrest
Culture pH levels demonstrate an inverse relationship between lactate accumulation and glycosylation quality; while lower pH controls lactate, it can negatively impact sialylation and galactosylation profiles.
1 claimspH 6.8-7.0: Significantly reduces lactate accumulation and ammonium production compared to pH 7.2
pH < 6.9: Risk of reduced sialylation content (N-glycan quality) despite productivity gains
High osmolality, resulting from concentrated feed strategies or specific salt additions, enhances specific productivity (qP) but induces apoptosis and reduces growth rates.
1 claimsOsmolality > 400 mOsm/kg: Increases specific antibody production rate but reduces specific growth rate
Hyperosmotic stress: Triggers distinct metabolic shifts favoring protein synthesis over biomass accumulation
Dissolved Oxygen (DO) setpoints generally support robust growth within a broad range (20-50%), but hypoxic conditions can alter product quality attributes.
1 claimsDO 30-50%: Standard operating range with minimal impact on VCD or titre
Hypoxic conditions (low DO): Can increase lactate production and alter glycosylation patterns (e.g., fucosylation levels)
Sodium Butyrate supplementation acts as a potent histone deacetylase inhibitor that increases titre at the cost of cell viability, with effects varying significantly by cell line sensitivity.
1 claimsButyrate addition (0.5-2 mM): Increases specific productivity (qP) but induces cell cycle arrest (G0/G1 phase)
Timing: Early addition halts growth immediately; late addition maximizes titre
Biphasic temperature shifts (lowering from 37°C to 30-33°C mid-run) increase final product titer by 40-100% despite significantly suppressing cell proliferation and lowering Peak VCD.
2 claimsStandard bioprocess assumptions equate higher biomass (VCD) with higher total product yield. The evidence challenges this by showing that arresting cell division (G1 phase) redirects metabolic energy from replication to protein synthesis and extends culture longevity, effectively decoupling growth from productivity.
Permitting pH to drift downward (to 6.8-6.9) in the late production phase triggers lactate consumption and extends viability, whereas maintaining a strict setpoint of 7.2 often leads to toxic lactate accumulation and earlier culture decline.
2 claimsTighter process control (strict pH set-points) is conventionally viewed as superior for consistency. Surprisingly, 'relaxed' control that mimics physiological acidification is more effective because it forces a metabolic switch (Warburg effect reversal) where cells utilize lactate as an energy source rather than producing it.
Hyperosmotic stress (400-500 mOsm/kg) increases specific productivity (qp) by up to 2-fold, but this yield gain is frequently offset by reduced sialylation and compromised glycan occupancy.
2 claimsWhile stress was expected to reduce overall performance, the specific upregulation of protein secretion machinery was unexpected. However, the surprise lies in the distinct trade-off: the cell produces more protein, but the intracellular transit time decreases, leaving insufficient time for complete glycosylation (specifically sialylation).
Absence of specific quantitative correlations between physicochemical parameters (pH, Temperature, DO) and glycosylation profiles
Glycosylation is a Critical Quality Attribute (CQA) determining drug safety and efficacy. The provided evidence map lacks data linking process shifts (e.g., temperature downshifts or pH ranges) to specific glycan structures (e.g., galactosylation or afucosylation levels), preventing the development of control strategies for product quality.
Lack of evidence linking Feeding strategies and Osmolality dynamics to Titre and Viable Cell Density (VCD)
Feeding regimens and osmolality control are the primary drivers for extending culture longevity and maximizing volumetric productivity. Without documented limits or optima for these inputs, practitioners cannot design fed-batch processes that balance high titre with sustained cell viability.
Missing comparative analysis of parameter sensitivity across different CHO cell lineages and product types
The research goal explicitly requests differentiation by cell line (e.g., CHO-K1 vs. CHO-DG44) and product type (mAb vs. fusion protein). The current lack of stratified evidence implies a 'one-size-fits-all' approach, which is risky given that different lineages possess distinct metabolic profiles and stress responses.
Undefined multivariate interactions between upstream parameters affecting process performance
Bioprocess parameters often interact non-linearly (e.g., the effect of pH may change depending on the temperature setpoint). The absence of evidence regarding these interaction effects prevents the establishment of a robust Design Space or the effective application of Quality by Design (QbD) principles.
| Document | Claims |
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An Overview of the Various Appropriate Types of Cell Lines for the Production of Monoclonal AntibodiJournal of Pharmaceutical Investigation
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An unconventional uptake rate objective function approach enhances applicability of genome-scale mod2019
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CO2 – Intrinsic Product, Essential Substrate, and Regulatory Trigger of Microbial and Mammalian Prod2015 · Frontiers in Bioengineering
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Cell-culture growth conditions resulting in the oxidation of a recombinant antigen-binding fragment2019 · Bioresour. Bioprocess.
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Decision Letter 0This research article focuses on refining a Genome-Scale Metabolic Model (GSMM) for CHO cells to accurately predict intracellular fluxes. Accurate flux prediction is a necessary step for linking...
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Cell Line Techniques and Gene Editing Tools for Antibody Production A Review2018 · Frontiers in Pharmacology
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Cellular and Molecular Engineering of Glycan Sialylation in Heterologous Systems2021
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Challenges and Emerging Technologies in Biomanufacturing of Monoclonal Antibodies (mAbs)This document is a review chapter focused on challenges and emerging technologies in CHO cell biomanufacturing of monoclonal antibodies (mAbs). It specifically mentions upstream processing,...
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Characterization of Monoclonal Antibody Glycan Heterogeneity Using Hydrophilic Interaction Liquid ChThe document, although focused on developing an analytical method (HILIC-MS) for glycosylation, explicitly applies this method to address research questions directly matching the goal: 1)...
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Characterization of Neutralizing Human Anti-Tetanus Monoclonal Antibodies Produced by Stable Cell Li2022
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Characterization of low temperature response and cyclin A isoforms in Chinese hamster ovary (CHO) ceProf. Dr.
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Characterization of metabolic responses, genetic variations, and microsatellite instability in ammonThe document investigates the effect of ammonia stress (a critical upstream metabolite/process parameter closely linked to feeding and pH) on CHO cell physiology and genomic integrity. The background...
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Characterization of the mechanism of action of spin-filters for animal cell perfusion cultures2006
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Charakterisierung des N-Glykosylierungsprofils der neuen Entenzelllinie AGE1.CR und die gerichtete MProf. Dr.
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Chemically modified cysteine in CHO fed-batch processes and impact on the specific productivityThis experimental study uses CHO cells and investigates the relationship between an upstream process parameter (feeding strategy, specifically the use of chemically modified L-cysteine/L-tyrosine...
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Chinese Hamster Ovary Cell Line Development via Imaging Flow Cytometry and Directed EvolutionThe document focuses on CHO cell line development and directed evolution to increase productivity (titre) for various molecules (mAbs). Although the primary independent variables are intrinsic...
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Cholesterol and fatty acids regulate cysteine ubiquitylation of ACAT2 through competitive oxidation.The document investigates CHO cells and studies the effect of upstream components (sterols and fatty acids, related to feeding/media) on cellular state (ACAT2 protein stabilization, ROS levels)....
3 claims
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Cleavage-Independent HIV-1 Trimers From CHO Cell Lines Elicit Robust Autologous Tier 2 Neutralizing2018 · Frontiers in Immunology
The document describes the development and characterization of a stable CHO cell line (CHO-M) for recombinant protein production, reporting specific product outcomes: high yield (~1.8 g/L, addressing...
3 claims
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Clustering and curation of electropherograms an efficient method for analyzing large cohorts of capi2020 · Org. Chem.
The document, although focused on an analytical method (CE-LIF data curation), is explicitly applied to datasets generated during 'bioreactor optimization' and 'process development' studies. The goal...
3 claims
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Coagulation factor IX analysis in bioreactor cell culture supernatant predicts quality of the purifi2021
The document investigates how 'bioreactor operating conditions' (upstream parameters) impact recombinant protein production (yield/titre) and specific product outcomes, namely post-translational...
3 claims
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Combining lipoic acid to methylene blue reduces the Warburg effect in CHO cells From TCA cycle activ2020 · PLOS ONE
The document investigates the relationship between an upstream process parameter (chemical addition/feeding strategy, using lipoic acid and methylene blue) and a key product outcome (monoclonal...
3 claims
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Compartment-specific metabolome labeling enables the identification of subcellular fluxes that may s2021
The document investigates metabolic flux patterns (using 13C MFA) in IgG1-producing CHO cells and directly relates specific fluxes (e.g., cytosolic malic enzyme) to cell-specific IgG1 productivity....
3 claims
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Comprehensive modeling of cell culture profile using Raman spectroscopy and machine learning2023
The document focuses on developing a comprehensive, automated method using Raman spectroscopy and machine learning to continuously monitor numerous components (metabolites, medium components, cell...
3 claims
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Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics2020
The study utilizes the Chinese Hamster Ovary (CHO) cell system for protein expression. It directly addresses product outcomes (expression/titre and glycosylation), noting that improved TCR stability...
2 claims
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Construction of a Public CHO Cell Line Transcript Database Using Versatile Bioinformatics Analysis P2014 · PLOS ONE
The document focuses on constructing a CHO cell line transcript database derived from cells grown under 'various culture conditions' and different cell lines. While the paper's focus is...
1 claim
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Construction of a novel kinetic model for the production process of a CVA6 VLP vaccine in CHO cells2023
The document focuses on CHO cell culture and explicitly investigates the relationship between an upstream process parameter (pH shift strategies) and process outcomes (cell growth/VCD, product...
3 claims
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Continuous Feeding Reduces the Generation of Metabolic Byproducts and Increases Antibodies Expressio2021
This experimental study focuses specifically on CHO-K1 cells and directly investigates the documented relationship between an upstream process parameter (feeding strategy) and product outcomes...
3 claims
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Controllability Analysis of Protein Glycosylation in Cho Cells2014 · PLOS ONE
The document specifically analyzes the relationship (via a process gain matrix and controllability assessment) between upstream process variables/operating conditions and the product outcome (protein...
3 claims
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Cysteine metabolic engineering and selective disulfide reduction produce superior antibody-drug-conj2022
The study details manipulating Cysteine availability/metabolism (a feeding/upstream process parameter) in CHO cells to control the modification status (TNB-capping) and resulting product...
3 claims
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Cyclin and DNA Distributed Cell Cycle Model for GS-NS0 Cells2015
The document develops a sophisticated population balance model (PBM) to link cellular state (cyclins/DNA) and 'stimulatory/inhibitory factors' in the medium to cell growth (VCD) and antibody...
3 claims
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Data-driven modeling of the degradation of recombinant proteins in Chinese hamster ovary cellsProf. Dr.
The document is a PhD thesis focusing on data-driven modeling and experimental study of recombinant protein degradation in CHO cell cultures. Degradation directly impacts product outcome...
3 claims
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Deciphering Metabolic Pathways in High-Seeding-Density Fed-Batch Processes for Monoclonal Antibody P2024
The document investigates the correlation between an upstream parameter (feeding strategy, specifically asparagine addition and bolus medium) and a key product outcome (final titers/productivity) in...
3 claims
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Deciphering Metabolic Pathways in High Seeding Density Fed-Batch Processes for Monoclonal Antibody PThe document investigates CHO cell fed-batch processes for mAb production (product type). It uses computational modeling based on experimental data to explore the relationship between upstream...
3 claims
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Decision Letter 02020 · PLOS ONE
The document is an experimental study using CHO cells to correlate an upstream process modification (addition of metabolic effectors, which is a type of feeding/media strategy) with a product outcome...
3 claims
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Decision Letter 12020 · PLOS ONE
The study investigates the effect of media supplements (lipoic acid, methylene blue, representing a feeding parameter) on CHO cell performance, specifically measuring maximum mAb production (titre),...
3 claims
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Decision Letter 2The document directly investigates the relationship between specific upstream process parameters (temperature decrease, chemical supplementation/feeding parameter NaBu) and product outcomes (enhanced...
3 claims
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Decision Modeling for understanding and engineering metabolism — R0 PR5The document reviews the principles of metabolic modeling and engineering (systems biology). These mathematical and computational methods are critical tools used to analyze, understand, and predict...
1 claim
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Decision Letter 22021 · PLOS ONE
The study directly investigates the relationship between an upstream intervention (addition of a chemical compound related to media/feeding) and key product outcomes (monoclonal antibody titre, cell...
3 claims
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Degradation Products of Tryptophan in Cell Culture Media Contribution to Color and Toxicity2021 · Toxicity. Int.
The document studies the effect of an upstream parameter (media component stability, driven by temperature) on CHO cell performance. It specifically tests the toxicity of tryptophan degradation...
2 claims
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Design of a stable cell line producing a recombinant monoclonal anti-TNFα antibody based on a CHO ceThe document focuses on developing a stable CHO cell line and maximizing productivity (titre >1 g/L) using DHFR-mediated gene amplification. While the primary scope is cell line development, it is...
2 claims
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Designed Amino Acid Feed in Improvement of Production and Quality Targets of a Therapeutic Monoclona2015 · PLOS ONE
The document directly addresses the research goal by studying the relationship between an upstream process parameter (designed amino acid feeding) and key product outcomes (titre and N-glycan...
3 claims
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Designing Robust Biotechnological Processes Regarding Variabilities Using Multi-Objective Optimizati2022
The document focuses on using multi-objective optimization (Bayes optimization/Gaussian processes) for upstream biopharmaceutical processes (seed train design). The objectives being optimized...
3 claims
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Designs and Characterization of Subunit Ebola GP Vaccine Candidates Implications for Immunogenicity.The document details the production of a recombinant glycoprotein (Ebola GP) in CHO cells, reporting specific culture parameters (37°C, fed-batch methodology, specific media) and resulting product...
3 claims
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Developing a medium combination to attain similar glycosylation profile to originator by DoE and clu2021
The document is an experimental study using CHO cells to optimize the relationship between upstream process parameters (basal medium, feed medium, galactose addition/feeding strategy) and product...
3 claims
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Development and evaluation of a new, specially tailored CHO media platformThe document investigates the effect of an upstream parameter (new media platform/feeding) on key product outcomes (VCD, product titer, and glycosylation) across multiple CHO cell lines (DG44, GS,...
2 claims
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Development of a Clonal and High-Yield Mammalian Cell Line for the Manufacturing of a Hyperactive Hu2024
The document details the development of a stable TurboCell™ CHO-K1-based cell line and the establishment of a 'robust bioprocess strategy' for manufacturing a recombinant protein. Studies focused on...
3 claims
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Development of a Stable MGAT1− CHO Cell Line to Produce Clade C gp120 With Improved Binding to Broad2018 · Frontiers in Immunology
The study focuses directly on CHO cell culture, documenting product outcomes (titre/productivity: 1.2 g/L) and product quality (glycosylation/glycoform heterogeneity) for a specific cell line...
3 claims
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Development of a fluorescent reporter system for monitoring ER stress in Chinese hamster ovary cells2017 · PLOS ONE
The document studies CHO cell productivity (titre) in fed-batch cultures and develops an ER stress index (ERSI) shown to be a 'good predictor of productivity.' The text explicitly states that ER...
3 claims
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Development of a scalable single process for producing SARS-CoV-2 RBD monomer and dimer vaccine antiThe document describes an experimental study using CHO-K1 cells to produce a therapeutic protein (SARS-CoV-2 RBD). It specifically identifies and links upstream process parameters (e.g., copper...
3 claims
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Development of a serum free medium for HUMIRA sup ® sup biosimilar by design of experiment approacheThis is an experimental study on CHO cells producing a mAb. It uses Design of Experiment (DoE) to determine the effect of upstream parameters (serum-free medium component concentrations and initial...
3 claims
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Development of an intensified fed-batch production platform with doubled titers using N-1 perfusion2020 · Bioresour. Bioprocess.
The document is an experimental study using CHO cells that investigates the impact of upstream process parameters (temperature shift optimization, feeding strategies, and cell density management) on...
3 claims
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Development of eukaryotic cell-free systems based on Chinese hamster ovary cells for the productionProf. Dr.
Although the document focuses on cell-free systems, the abstract explicitly states that the research addressed the optimization and upscaling of CHO cell cultivation parameters (e.g., transitioning...
3 claims
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Differential effect of culture temperature and specific growth rate on CHO cell behavior in chemosta2014 · PLOS ONE
The document experimentally investigates the relationship between upstream process parameters (culture temperature and specific growth rate, linked to feeding/dilution rate) and a product outcome...
3 claims
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Differential protein expression following low temperature culture of suspension CHO-K1 cells2008
The document directly investigates the effect of an upstream process parameter (temperature shift from 37°C to 31°C) on CHO cell cultures (CHO-K1). It measures the resulting changes in viable cell...
3 claims
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Direct analysis of mAb aggregates in mammalian cell culture supernatantThe document investigates mAb aggregation, a critical product outcome/quality attribute (PQA), specifically in CHO cell cultures. Although primarily a methodology article, it explicitly discusses how...
3 claims
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Dual formation mechanisms of acidic variants in cysteine-engineered antibodies and strategies for thThe document directly investigates the relationship between upstream process parameters (modulating temperature and pH, and supplementing L-cysteine/feeding strategy) and a critical product quality...
3 claims
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Dynamic Modeling of CHO Cell Metabolism Using the Hybrid Cybernetic Approach With a Novel Elementary2020 · Frontiers in Bioengineering
The document develops a dynamic metabolic modeling methodology (Hybrid Cybernetic Modeling) specifically for CHO-S cell cultures. The stated purpose of the modeling is to identify relevant 'culture...
3 claims
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Dynamic Optimisation of Fed-Batch Bioreactors for mAbs Sensitivity Analysis of Feed Nutrient Manipul2023
The document investigates the dynamic optimization and sensitivity analysis of upstream process parameters (feeding trajectories/substrate manipulation: Glucose, Glutamine, Asparagine) in GS-CHO cell...
3 claims
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Effect of Sialic Acid on Mammalian Cell Culture and Protein Expression A Potential Productivity Enha2020
The document studies the effect of an upstream process parameter (Sialic Acid addition/medium composition) on key product outcomes (VCD, antibody titer) and product quality attributes (N-linked...
3 claims
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Effect of Temperature Downshift on the Transcriptomic Responses of Chinese Hamster Ovary Cells Using2016 · PLOS ONE
The research article directly studies the relationship between an upstream process parameter (temperature downshift) and product outcomes (increased specific productivity/titre, and transcriptomic...
3 claims
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Effect of amino acid limitation and supplementation in Chinese hamster ovary fed-batch culturesThis thesis directly investigates the effect of specific upstream process parameters (amino acid limitation/feeding) on key product outcomes (cell growth/VCD, antibody productivity/titre, and...
3 claims
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Expression of Recombinant Antibodies2013
The review covers recombinant antibody expression, explicitly mentioning mammalian cell lines (CHO) as the primary therapeutic production system and highlighting the importance of glycosylation (a...
3 claims
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Effect of different cell culture media on the production and glycosylation of a monoclonal antibody2025
The study directly investigates the relationship between an upstream process parameter (cell culture media composition and feeding strategy) and key product outcomes (titer, cell growth/VCD, and...
3 claims
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Effect of iron sources on the glycosylation macroheterogeneity of human recombinant IFN-γ produced bThe document investigates the relationship between an upstream process parameter (iron source/feeding strategy) and multiple specified product outcomes (VCD, titre/production rate, and glycosylation)...
2 claims
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Effects of autophagy-inhibiting chemicals on sialylation of Fc-fusion glycoprotein in recombinant CH2024
The document investigates the relationship between a process intervention (chemical inhibition of autophagy) and a key product outcome specified in the research goal: glycosylation (sialylation) in...
3 claims
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Effects of enzymatically-synthesized sialylated oligosaccharides on the N- and O-glycosylation of etThe document investigates the relationship between an upstream process parameter (supplementation/feeding of sialylated oligosaccharides) and a key product outcome (N- and...
3 claims
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Effects of process intensification on homogeneity of an IgG1 κ monoclonal antibody during perfusion2024
The document investigates the relationship between upstream process parameters (process intensification/high cell density targets in perfusion culture) and a product outcome (glycosylation/product...
3 claims
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Efficient Production of sTNFRII-gAD Fusion Protein in Large Quantity by Use of the Modified CHO-S Ce2014 · PLOS ONE
This experimental paper focuses on developing and optimizing a fed-batch culture system for a recombinant protein (sTNFRII-gAD) in CHO-S cells. The abstract explicitly mentions 'optimized the...
3 claims
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Employing active learning in the optimization of culture medium for mammalian cells2023
The document describes a methodology (Active Learning and GBDT) for optimizing culture medium composition (a crucial upstream process parameter related to feeding) based on cellular metabolic status...
3 claims
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Endoplasmic Reticulum-Associated rht-PA Processing in CHO Cells Influence of Mild Hypothermia and Sp2015 · PLOS ONE
The article experimentally studies the relationship between an upstream process parameter (Temperature/Mild Hypothermia) and cellular outcomes (rht-PA processing/degradation) in CHO cell cultures....
3 claims
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Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell prolif2023
The document investigates CHO cell productivity (titre, VCD) and specifically evaluates the performance of the engineered cells under different environmental/upstream process parameters, namely...
3 claims
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Engineering protein glycosylation in CHO cells to be highly similar to murine host cellsThe document is an experimental study focusing on CHO cell cultures and the critical product outcome of glycosylation. It explicitly mentions analyzing and selecting clones based on 'similar growth...
3 claims
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Enhanced recombinant protein production in CHO cell continuous cultures under growth-inhibiting condThe document investigates the relationship between an upstream process parameter (low temperature, a key parameter listed in the goal) and product outcomes (recombinant protein...
3 claims
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Enhanced understanding of protein glycosylation in CHO cells through computational tools and experimThis is a PhD thesis (a required source type) focused on CHO cells, specifically investigating the relationship between upstream process parameters (galactose and uridine feeding/supplementation) and...
3 claims
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Enhancement of adalimumab expression in CHO cells by antioxidant small molecule additives and the unThe document investigates how small molecule additives (a type of upstream process parameter/medium component related to feeding) affect product outcomes in CHO cells, specifically focusing on...
3 claims
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Enhancement of effector functions of anti-CD20 monoclonal antibody by increased afucosylation in CHO2022 · Journal of Genetic Engineering and Biotechnology
The document investigates the relationship between an upstream process parameter, osmolality (modulated by UMG feed additives), and a product quality attribute, glycosylation (specifically...
3 claims
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Enhancing Antibody-Specific Productivity Unraveling the Impact of XBP1s Overexpression and Glutamine2024
The document investigates the correlation between an upstream parameter (Glutamine availability/feeding) and product outcomes (specific productivity, metabolic byproducts) using experimental data....
3 claims
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Enhancing and stabilizing monoclonal antibody production by Chinese hamster ovary (CHO) cells with oThe document directly addresses the research goal by investigating the optimization of upstream process parameters (perfusion culture strategies, perfusion rates, and temperature reduction methods)...
3 claims
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Entwicklung einer API-Produktionsplattform mittels CRISPR Cas9 auf Basis einer CHO Zelllinie2018 · Prof. Dr.
The document, a PhD thesis on CHO cell line development for API production, uses fed-batch cultivation and measures VCD/titre (Section 5.2.2.12, 5.2.2.5, 3.3). These methods necessarily involve...
3 claims
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Entwicklung einer Affinitätstechnologie für die Aufreinigung rekombinanter Proteine aus Säugetierzel2004 · Journal of Chromatography B
The document investigates CHO cell cultivation (specifically CHO t-PA and CHO hGH lines) and analyzes upstream process impacts. Section 4.2.5 is explicitly titled 'Einfluss der...
3 claims
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Establishment of fast-growing serum-free immortalised cells from Chinese hamster lung tissues for bi2020
The document investigates a Chinese hamster derived cell line (CHL-YN) and compares its performance (growth, VCD, IgG1 titre, and glycosylation levels) to conventional CHO-K1 cells under serum-free...
3 claims
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Evaluating the impact of media and feed combinations on CHO cell culture performance and monoclonal2025
The document is an experimental study in CHO cell culture, directly investigating the relationship between an upstream process parameter (feeding/media composition) and key product outcomes (viable...
3 claims
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Evaluation and use of disaccharides as energy source in protein-free mammalian cell culturesThe document investigates the relationship between an upstream process parameter (carbon source / feeding strategy) and a documented product outcome (Viable Cell Density, VCD) in CHO cell lines...
3 claims
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Evaluation of feed strategy for high quality biosimilar IgG production in CHO cell fed-batch process2021 · Bilim. Derg.
The document is an experimental study in CHO cells investigating the relationship between an upstream process parameter ('Feed Strategy'/'Feeding') and key product outcomes ('product titer' and...
3 claims
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Evaluation of the advanced micro-scale bioreactor (ambr™) as a highthroughput tool for cell cultureThe document describes an experimental study using CHO cells where two key upstream process parameters (pH and feeding/feed rate) are varied using a factorial design, and the resulting impact on...
3 claims
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Evaluation of transfection methods for transient gene expression in Chinese hamster ovary cells2013
The document investigates optimization strategies in CHO cells for protein production (titre/EGFP expression) and cell growth (VCD). Specifically, it studies the effect of a temperature shift (37°C...
3 claims
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Evidence for co-translational misincorporation of non-canonical amino acid hydroxyproline in recombi2020 · PLOS ONE
The document investigates the relationship between an upstream process parameter (proline content in the cell culture media, a 'feeding' variable) and a product outcome (hydroxyproline...
3 claims
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Explainable AI for CHO cell culture media optimization and prediction of critical quality attribute2024
The document investigates the relationship between upstream parameters (media components/metal ion concentrations, which relate to feeding/media strategy) and a product outcome (Critical Quality...
3 claims
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Exploration of linear and interpretable models for quantification of cell parameters via contactlessThe document focuses on developing an analytical method (SWIR sensing) for contactless quantification of key bioprocess parameters, including Viable Cell Density (VCD) and critical metabolites...
2 claims
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Exploring metabolic effects of dipeptide feed media on CHO cell cultures by in silico model-guided f2024
The document directly investigates the relationship between an upstream process parameter (feeding/concentration of dipeptide in feed media) and product outcomes (IgG titer, specific cell...
3 claims
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Monoclonal Antibodies4468
The table of contents includes the article titled, 'Controlling the Glycosylation Profile in mAbs Using Time-Dependent Media Supplementation,' which directly addresses the research goal by linking an...
2 claims
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Exploring synthetic biology for the development of a sensor cell line for automated bioprocess contr2022
The document is highly relevant as it focuses on two key upstream process parameters (DO/hypoxia and osmolality) in CHO cell cultures, which are central to the research goal. Although the main output...
3 claims
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Exploring the limits of conventional small-scale CHO fed-batch for accelerated on demand monoclonal2021
This research paper directly investigates the relationships between upstream process parameters (temperature shift, pH shift, feeding strategy, high cell density/IVCD management, and DO levels) and...
3 claims
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Expression and Purification of Biologically Active Human Bone Morphogenetic Protein-4 in Recombinant2017 · Microbiol. Biotechnol.
The document is an experimental study detailing the development of a production process for rhBMP-4 in rCHO cells using a 7.5-L bioreactor. It explicitly mentions and investigates the use of specific...
3 claims
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Expression of Human Erythropoietin Containing 2 Additional N-Link in CHO-K1 Cells under Different CuJournal of Cancer Chemoprevention
The document investigates the relationship between upstream CHO cell culture parameters (seeding density, incubation time, media type, FBS concentration) and a product outcome (recombinant protein...
3 claims
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Factors Affecting the Expression of Recombinant Protein and Improvement Strategies in Chinese HamsteThis is a comprehensive review article on factors affecting recombinant protein expression (yield/titre and quality/PTMs like glycosylation) and improvement strategies in CHO cells. While the visible...
3 claims
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Fast and long-lasting immune response to S-trimer COVID-19 vaccine adjuvanted by PIKA2021
Although the primary focus is vaccine efficacy, the document explicitly describes the production of the S-trimer antigen using 'monoclonal CHO cells' in a 'fed batch serum-free cell culture process...
1 claim
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Fc-GDF15 glyco-engineering and receptor binding affinity optimization for body weight regulation2021
The document focuses heavily on optimizing product outcomes, specifically **glycosylation** (glyco-engineering) and **titer/yield** in a therapeutic Fc-fusion protein. This addresses two key required...
3 claims
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From Cell Clones to Recombinant Protein Product Heterogeneity in Chinese Hamster Ovary Cell Systems2025 · Systems. Int.
This review summarizes developments in CHO cell expression systems, specifically addressing product heterogeneity and yield. It explicitly mentions 'optimization of culture media, cell culture...
3 claims
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From Observational to Actionable Rethinking Omics in Biologics ProductionThis review focuses on applying omic and systems biology approaches to optimize CHO cell bioprocesses, explicitly targeting improved titer and product quality (PQ). The methodologies discussed...
3 claims
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From Shallow to Deep Bioprocess Hybrid Modeling Advances and Future PerspectivesThis document reviews the application of Hybrid Neural Networks (HNNs) and deep learning to bioprocesses, specifically mentioning 'animal cells cultures' and the biopharma sector. It discusses...
2 claims
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From Spatial-Temporal Multiscale Modeling to Application Bridging the Valley of Death in Industrial2023
This review paper focuses on advanced modeling techniques (mechanistic, data-driven, hybrid) and AI applications for bioprocess development, optimization, and control in industrial biotechnology....
2 claims
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Functional Trimeric SARS-CoV-2 Envelope Protein Expressed in Stable CHO CellsThe document focuses on expressing a complex glycoprotein (SARS-CoV-2 Spike protein) in stable CHO cells. Crucially, it discusses optimizing the bioprocess, explicitly stating that 'Final harvest...
3 claims
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GMP Manufacturing and Characterization of the HIV Booster Immunogen HxB2.WT.Core-C4b for Germline Ta2025
The document describes the optimization of a CHO cell culture process for producing a recombinant protein (HIV immunogen). It explicitly mentions refining key upstream process parameters (pH,...
3 claims
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Gene editing in CHO cells to prevent proteolysis and enhance glycosylation Production of HIV envelop2020 · PLOS ONE
The document investigates improvements in a key product outcome, glycosylation, by developing and characterizing novel CHO cell lines (C1s-/- MGAT1- CHOK1). While it focuses on genetic modification...
3 claims
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Generating aldehyde-tagged antibodies with high titers and high formylglycine yields by supplementinThe study analyzes the relationship between supplementing culture media with copper (an upstream process parameter/feeding) and product outcomes, specifically antibody titer and a critical product...
2 claims
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Generation and Characterization of a Bivalent HIV-1 Subtype C gp120 Protein Boost for Proof-of-ConcePLOS ONE
The study uses CHO cells to produce a therapeutic protein (gp120) and provides detailed analytical characterization of the product, specifically focusing on glycan content and occupancy...
2 claims
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Genome-scale modeling of Chinese hamster ovary cells by hybrid semi-parametric flux balance analysis2022
The document, while focused on modeling (Hybrid FBA), uses an experimental CHO cell dataset derived from 21 reactor experiments with measured exchange fluxes to evaluate and validate the model. The...
3 claims
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Genome-scale reconstructions of the mammalian secretory pathway predict metabolic costs and limitati2020
The document focuses on genome-scale metabolic modeling of CHO cells (iCHO2048s) to predict product outcomes (maximum productivity/titre) and quantify the metabolic costs related to protein...
3 claims
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Getting CD19 Into Shape Expression of Natively Folded “Difficult-to- Express” CD19 for Staining and2020 · Frontiers in Bioengineering
The document is an experimental study using CHO-K1 cells and directly examines the relationship between an upstream process parameter (media supplementation with chemical chaperones like Valproic...
3 claims
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Glucose withdrawal-induced Chinese hamster ovary cells apoptosis and its potential role in process rThis is an experimental study using CHO cells that investigates the relationship between upstream parameters (glucose withdrawal/feeding, temperature) and cell viability (VCD) and product titer. This...
3 claims
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Glycosylation Flux Analysis of Immunoglobulin G in Chinese Hamster Ovary Perfusion Cell CultureThe document directly addresses the relationship between process parameters/metabolites (ammonia concentration, perfusion rate) and product outcomes (IgG titer, VCD, glycosylation/galactosylation...
2 claims
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High-Level Production of scFv-Fc Antibody Using an Artificial Promoter System with Transcriptional P2023
The document investigates the correlation between an upstream process parameter (culture temperature: 37°C vs 33°C) and product outcomes (titre/concentration, specific productivity, and glycosylation...
2 claims
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High-throughput process development from gene cloning to protein production2023
This review focuses on high-throughput process development for recombinant protein production, which includes optimizing process-related parameters using platforms like MTPs and microbioreactors....
1 claim
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High glucose and low specific cell growth but not mild hypothermia improve specific r-protein producPLOS ONE
The document is a research article focusing on CHO cell cultures (Chinese hamster ovary cells) and experimentally investigates the relationship between core upstream process parameters (Temperature...
3 claims
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Host Cell Proteins in Biologics Manufacturing The Good, the Bad, and the Ugly2017
The document focuses on Host Cell Proteins (HCPs) and Downstream Processing (DSP), but explicitly links the findings back to CHO Upstream Processing (USP) optimization, stating the data serves for...
2 claims
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Hybrid Modeling for On-Line Fermentation Optimization and Scale-Up A Review2024
This is a review article on hybrid modeling for fermentation optimization and scale-up. It specifically mentions that mammalian cell culture, particularly for monoclonal antibodies (CHO cell domain),...
1 claim
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Hybrid Modelling and Multi- Parametric Control of Bioprocesses5354 · Name. Year.
The document is a collection of articles focusing on hybrid modeling and multi-parametric control of bioprocesses, which is the methodology needed to establish parameter-outcome relationships (the...
2 claims
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Hyperosmolality in CHO cell culture effects on the proteome2022
The document directly investigates the effect of a key upstream process parameter (hyperosmolality) on CHO cell physiology and culture characteristics (cell size, VCD/proliferation, mitochondrial...
3 claims
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Impact of Acetylated and Non-Acetylated Fucose Analogues on IgG Glycosylation2019
This document describes an experimental study using CHO cells to correlate a modification in the feeding strategy (addition of fucose analogues) with key product outcomes (glycosylation, specifically...
3 claims
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Impact of fed-batch process intensification on the productivity and product quality of two CHO cell2024
The document directly addresses the research goal by studying the impact of an upstream process parameter (process intensification via High Inoculation Density fed-batch, which affects VCD/VCC and...
3 claims
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Improved fed-batch bioprocesses using chemically modified amino acids in concentrated feedsThis document studies the relationship between upstream process parameters (feeding strategy, specifically the use of modified amino acids enabling neutral pH feeding) and critical product outcomes...
2 claims
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untitled2006
The document measures key product outcomes (recombinant glycoprotein yield and glycosylation profiles) and compares them across different CHO cell lines (parental vs. engineered) under a fed-batch...
1 claim
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Improving Selectivity in the Chromatographic Analysis of Monoclonal Antibodies (mAbs) Through the UsThe document, focused on biopharmaceutical analysis, contains articles describing analytical methods (e.g., Multi-Attribute Method, online HPLC–HRMS) explicitly used for monitoring Product Quality...
2 claims
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Improving product quality and productivity of an antibody-based biotherapeutic using inverted frustoThe document is an experimental study using CHO cells and explicitly reports the relationship between process conditions (bioreactor type, which affects mixing/shear/gas transfer, influencing DO/pH...
3 claims
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In Situ Monitoring of Intracellular Glucose and Glutamine in CHO Cell Culture2012 · PLOS ONE
The document focuses on in situ monitoring of key intracellular metabolites (glucose and glutamine) in CHO cell culture during batch and fed-batch processes. Monitoring nutrient utilization rates is...
3 claims
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In situ FRET Biosensors for the in vivo measurement of important metabolites during cell cultureThe document is a PhD thesis focusing on CHO cell cultures (Chinese Hamster Ovary cells) and investigates the correlation between internal metabolite levels (driven by upstream parameters like...
3 claims
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Inclusion of maintenance energy improves the intracellular flux predictions of CHOThe document focuses on improving Genome-Scale Metabolic Models (GSMM) for CHO cells to accurately predict intracellular fluxes. Accurate flux prediction is a necessary intermediary step for linking...
3 claims
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Induction of heat shock protein expression in SP2 0 transgenic cells and its effect on the productioPLOS ONE
Although the primary experiment uses SP2/0 cells, the document directly investigates the relationship between upstream parameters (heat shock/temperature stress, chemical induction) and product...
3 claims
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Influence of cell-line and process conditions on the glycosylation of recombinant proteins2002
The document directly studies the influence of cell line (CHO clones) and upstream process conditions (specifically temperature) on a critical product outcome (glycosylation) for pharmaceutically...
3 claims
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Influence of cell culture media and feed supplements on cell metabolism and quality of IgG producedThe document directly addresses the research goal by studying the influence of upstream process variables (media/feed composition, which is part of 'feeding,' and measured 'osmolality') on key...
2 claims
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Integration Approaches to Model Bioreactor Hydrodynamics and Cellular Kinetics for Advancing Bioproc2024
This review article discusses the integration of bioprocess modeling approaches (CFD-CRK) specifically to relate process parameter gradients (substrate, DO, pH, osmolality) in large-scale bioreactors...
2 claims
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Integration of Time-Series Transcriptomic Data with Genome-Scale CHO Metabolic Models for mAb Engine2020
The document directly addresses CHO cell culture and the link between changing environmental conditions (e.g., pH, altered substrate availability, reflective of process parameters) and physiological...
2 claims
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Integrative overview of antibodies against SARS-CoV-2 and their possible applications in COVID-19 pr2021
The document reviews the challenges in producing therapeutic antibodies by 'cell factories' (likely CHO cells) at an industrial level, emphasizing the need to ensure 'quality, efficacy, and safety.'...
3 claims
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Intracellular Profiling for Biopharmaceutical Cultivation ProcessesIng. Univ.
Doctoral thesis focusing on the characterization and optimization of mAb producing CHO cell cultures. The work explicitly studies the correlation between process conditions (fed-batch vs. perfusion,...
3 claims
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Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch cultu2021
The document describes an experimental study in CHO cells investigating the influence of an upstream process parameter (hydrostatic pressure) on key product outcomes, specifically viable cell...
3 claims
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Investigation of the interactions of critical scale-up parameters (pH, pO2 and pCO2) on CHO batch pe2016
The document investigates the interactions of critical upstream process parameters (pH, pO2/DO, pCO2) on CHO cell performance (specific productivity) and critical quality attributes (CQAs) such as...
3 claims
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Investigation of the influence of large scale occurring pH heterogeneities on CHO cellsUniv. Prof. Dipl.
This experimental thesis investigates the influence of an upstream process parameter (pH heterogeneity/gradient) on CHO cell productivity (titre, growth behavior) and specific rates (VCD, metabolite...
3 claims
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Key Challenges in Designing CHO Chassis Platforms2020
This is a review focused on optimizing CHO cell lines (chassis platforms) to improve production performance, specifically addressing desired product titers and quality (including glycosylation)....
3 claims
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Life at the periphery what makes CHO cells survival talents2022
The document investigates the genomics and proteomics related to CHO cell survival and growth under bioprocess stress, explicitly mentioning tolerance to variations in pH and osmolality. This...
2 claims
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Low-cost food-grade alternatives for serum albumins in FBS-free cell culture mediaThe document investigates upstream process parameters (medium formulation/stabilization, which falls under 'feeding') and cell culture outcomes (proliferation/VCD) using the required system, CHO...
2 claims
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Low Level Sequence Variant Analysis of Recombinant Proteins An Optimized Approach2012 · PLOS ONE
The document, while focusing on analytical methods for detection, explicitly discusses product outcomes (sequence variants/misincorporations) in CHO cells and links them to upstream process...
3 claims
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MEQ Protein #4 MEQ Cytokine for Immune Modulation2025 · Journal of Infectious Diseases and Research
The document discusses the production of a recombinant cytokine in CHO cells, specifically mentioning optimization related to key upstream parameters (temperature, pH, nutrient supply/feeding) and...
1 claim
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Main Quality Attributes of Monoclonal Antibodies and Effect of Cell Culture Components2016 · Iran. Biomed.
This review article directly addresses the research goal by examining the relationship between upstream process parameters (culture media components, pH, temperature, dissolved oxygen, CO2, feeding...
3 claims
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Macroscopic modeling of mammalian cell growth and metabolism2015
The mini-review discusses modeling strategies (stoichiometric and kinetic) used to understand and simulate macroscopic behavior in CHO cell cultures. It explicitly covers the need to identify...
2 claims
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Macro- and Micro-Heterogeneity of Natural and Recombinant IgG Antibodies2019
This document is a review focusing heavily on characterizing product outcomes, specifically glycosylation (a key variable in the research goal) and other post-translational modifications (PTMs) that...
3 claims
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Mammalian Expression System and Improvisation for High Production2013 · Journal of Science and Research
This review article focuses on improving productivity (titre and VCD) and post-translational modifications (glycosylation/sialylation) in mammalian systems, particularly CHO cells, which aligns with...
3 claims
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Mammalian cell perfusion culture development for integrated continuous biomanufacturingProf. Dr.
The doctoral thesis focuses on CHO cell perfusion culture (mammalian cell type) and explicitly investigates the effect of 'operating points and media supplements' (upstream process parameters) on...
3 claims
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Mapping the molecular basis for growth related phenotypes in industrial producer CHO cell lines usinThe document investigates relationships between cell line phenotypes (VCD, which is a key product outcome) and specific cellular pathways in CHO cells. Although the methodology is focused on...
3 claims
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Manufacturing ultra-concentrated liquid feeds Transitioning the aqueous solubility barrier of the feThe document investigates CHO fed-batch cultivation, comparing how a change in the upstream parameter (feeding strategy, specifically ultra-concentrated DES feed) and the resulting increase in...
3 claims
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Medium and feed optimization for fed-batch production of a monoclonal antibody in CHO cells.The document investigates the relationship between upstream process parameters (feeding strategy, media composition, osmolality changes) and key product outcomes (MAb titer, VCD/cell count, average...
3 claims
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Metabolic characterization of a CHO cell size increase phase in fed-batch cultures2017
The document describes an experimental fed-batch CHO cell culture study. It explicitly documents the relationship between several listed upstream parameters (DO controlled at 40%, pH controlled at...
3 claims
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Metabolic dynamics and compartmentation in the central metabolism of Chinese hamster ovary cellsProf. Dr.
The document is a dissertation focused on experimental studies of Chinese hamster ovary (CHO) cell metabolism and metabolic flux analysis (MFA). It directly investigates the impact of an upstream...
3 claims
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Metabolic engineering of CHO cells for the development of a robust protein production platform2017 · PLOS ONE
The document investigates CHO cell culture performance under fed-batch conditions. It directly measures key output parameters listed in the research goal: VCD (cell density), Titer (enhanced antibody...
3 claims
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Metabolomics - a useful tool for prediction of protein production and processingThe document investigates CHO cells and the relationship between metabolic alterations (which are determined by upstream process parameters and feeding) and product processing/quality (specifically...
2 claims
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Microevolutionary dynamics of eccDNA in Chinese hamster ovary cells grown in fed-batch cultures unde2023
The study investigates the effect of an upstream process parameter (lactate stress, which results from metabolism and feeding conditions) on CHO cell performance in fed-batch culture. It explicitly...
3 claims
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Microevolutionary dynamics of eccDNA in Chinese hamster ovary cells grown in fed-batch cultures undeThis is an experimental study using CHO cells in fed-batch culture. It explicitly controls and monitors multiple upstream process parameters listed in the research goal (Temperature, pH, DO, Feeding...
2 claims
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Mild hypothermia upregulates myc and xbp1s expression and improves anti-TNFα production in CHO cellsPLOS ONE
The document directly investigates the relationship between an upstream process parameter (Temperature, using mild hypothermia: 37, 33, 31°C) and key product outcomes (anti-TNFα production/titre,...
3 claims
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Model-Based Design of Process Strategies for Cell Culture Bioprocesses State of the Art and New PersThe document discusses the framework (Quality by Design, DoE, and kinetic modeling) used to design and analyze cell culture bioprocesses, explicitly focusing on relating Critical Process Parameters...
2 claims
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Model-Based Methods in the Biopharmaceutical Process Lifecycle2017
This is an expert review on model-based methods (including data mining, DoE, and multivariate statistics like PLS/PCA) used in the biopharmaceutical process lifecycle. The goal is to find...
2 claims
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Model-based intensification of CHO cell cultures One-step strategy from fed-batch to perfusion.2022 · Frontiers in Bioengineering
The document describes a model-based and experimentally validated study using a CHO DG44 cell line to capture upstream bioprocess dynamics, specifically the relationship between operating modes...
3 claims
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Model Transferability and Reduced Experimental Burden in Cell Culture Process Development Facilitate2021 · Frontiers in Bioengineering
The document describes an experimental study in CHO cells investigating the impact of Critical Process Parameters (cultivation temperature and glucose concentration in the feed) on product outcomes...
3 claims
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Modeling, estimation, and control of glycosylation in monoclonal antibodies produced in CHO cellsThe document is a dissertation focused on 'MODELING, ESTIMATION, AND CONTROL OF GLYCOSYLATION IN MONOCLONAL ANTIBODIES PRODUCED IN CHO CELLS.' This directly addresses the research goal, which...
3 claims
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Modulating antibody N-glycosylation through feed additives using a multi-tiered approachThe document directly studies the relationship between upstream process parameters (feed additives, which fall under 'feeding') and product outcomes (glycosylation, titre, and VCD via cell density...
3 claims
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Modulation of N-Glycosylation in Bispecific Antibody Biosimilars through Combined ModulatorsThe document directly investigates the relationship between upstream parameters (culture media additives: galactose, Tris, MnCl2) and a key product outcome (N-Glycosylation, specifically...
3 claims
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Monitoring and modelling the glutamine metabolic pathway a review and future perspectives2023
This review focuses on monitoring and modeling glutamine metabolism using techniques like metabolomics and flux analysis (MFA/FBA). While the primary context examples are cancer cells, these...
3 claims
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Monitoring of Nutrients, Metabolites, IgG Titer, and Cell Densities in 10 L Bioreactors Using Raman2025
The document is an experimental study focusing on CHO cell culture and the real-time monitoring of key inputs (nutrients, metabolites like glucose, lactate) and key product outcomes (IgG Titer, VCD)....
3 claims
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Monoclonal Antibody-Directed Therapy4468
This is a compilation of articles focused on Monoclonal Antibody (mAb) manufacture, formulation, and quality attributes. The table of contents explicitly lists an article on 'Current Advancements in...
2 claims
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Multi-attribute monitoring (MAM) methodology for glycosylated subunit vaccinesThe document focuses on Multi-Attribute Monitoring (MAM), a critical analytical methodology used for high-resolution measurement of key product outcomes, specifically glycosylation and other PTMs....
3 claims
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Multidisciplinary Analysis of the Metabolic Shift to Lactate Consumption in CHO Cell Culture2012
The document is an experimental CHO cell culture thesis focusing on analyzing the metabolic shift related to lactate production/consumption. Lactate levels are a critical intermediate process...
3 claims
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Multiplex secretome engineering enhances recombinant protein production and purity2020
The document reports experimental findings on engineered CHO cell lines characterized in bioreactors. It directly addresses key product outcomes (increased mAb productivity/titer and improved growth...
3 claims
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Multivariate data analysis of capacitance frequency scanning for online monitoring of viable cell coThe document investigates Fed-Batch cultivations (likely CHO cells, standard bioprocess context) and compares outcomes (VCC, Viability) across different upstream parameters, specifically 'Altered...
2 claims
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N-Glycosylation modulators for targeted manipulation of glycosylation for monoclonal antibodies2025
The document directly studies the impact of upstream process parameters (media additives, which fall under feeding/media composition) on product outcomes (glycosylation, titer, and viability) in CHO...
3 claims
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Narrow Operating Space Based on the Inversion of Latent Structures Model for Glycosylation ProcessThe document focuses on developing a data-driven model to establish the relationship between process variables (input combination/operating conditions) and the resulting product quality attribute...
3 claims
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New high-throughput screening method for Chinese hamster ovary cell lines expressing low reduced monThe document investigates a critical product outcome (monoclonal antibody reduction, which affects yield and purity) in CHO cells and links it to both cell line characteristics and documented...
3 claims
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Optimization of Culture Conditions to Improve Follicle-Stimulating Hormone Production by CHO-DG44 Ce2024
The document is an experimental study on CHO cells, directly investigating the relationship between upstream process parameters (temperature shift, chemical supplementation/feeding agents like NaBu...
3 claims
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Optimization of the Process of Chinese Hamster Ovary (CHO) Cell Fed-Batch Culture to Stabilize Monoc2024
The document directly investigates the effect of an upstream process parameter (pH control strategy) on key product outcomes (titre and glycosylation/product quality metrics) in CHO cell fed-batch...
3 claims
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Optimization of the medium perfusion rate in a packed-bed bioreactor charged with CHO cells.2004
The document investigates the relationship between an upstream process parameter (medium perfusion rate, which is a key feeding parameter) and product outcomes (volumetric productivity/titre and...
3 claims
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Optimization of transient protein production by chemically transfected CHO suspension cellsProf. Dr.
The document is a PhD thesis abstract focusing on the optimization of transient protein production in CHO-K1 suspension cells. It explicitly investigates the relationship between upstream parameters...
3 claims
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Optimized Production of Virus-like Particles in a High-CHO-Cell-Density Transient Gene Expression Sy2025
This is an experimental optimization study using CHO cells for VLP production. It investigates how certain process parameters (cell density, transfection inputs like DNA/PEI ratio, and harvest time)...
3 claims
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Optimized platform medium and feed for rCHO cell lines using the CHEF1® expression systemThe document is an experimental study on rCHO cells optimizing feeding protocols (upstream process parameter) based on nutrient consumption rates. It reports the resulting impact on viable cell...
3 claims
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Optimizing the Production of gp145, an HIV-1 Envelope Glycoprotein Vaccine Candidate and Its Encapsu2023
The document describes optimizing the production of a glycoprotein (gp145) in Chinese Hamster Ovary (CHO) cells. It explicitly tests the relationship between an upstream parameter (pH adjustment from...
3 claims
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Over-expression of a Codon Optimized Yeast Cytosolic Pyruvate Carboxylase (PYC2) in CHO Cells for an2017 · Frontiers in Pharmacology
The document focuses on metabolic engineering in CHO cells (PYC2 overexpression) to mitigate lactate accumulation. This accumulation is directly linked to upstream process parameters (feeding...
3 claims
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Overexpression of MiR-32 in Chinese Hamster Ovary Cells Increases Production of Fc-fusion ProteinThe document investigates engineered CHO cells producing an Fc-fusion protein and measures key output variables (productivity/titre, cell growth, viability). While the manipulation is genetic (miR-32...
2 claims
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Oxygen Uptake Rate Soft-Sensing via Dynamic kLa Computation Cell Volume and Metabolic Transition Pre2019 · Frontiers in Bioengineering
The document investigates the correlation between an upstream parameter, Oxygen Uptake Rate (OUR, derived from DO/kLa), and a product outcome metric, Viable Cell Density (biomass/cell volume), within...
3 claims
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PID controls the forgotten bioprocess parameters2022
The document investigates CHO cell culture studies and explicitly links the control of critical upstream process parameters (pH, DO) via PID tuning to key product outcomes (robust cell growth,...
3 claims
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Perfusion-Based Antibody Production in the Ambr<sup>®< sup> 250 ModularProf. Dr. Ing.
The document describes experimental work with CHO cells producing monoclonal antibodies (mAb) in perfusion mode. It measures key outputs (VCD, volumetric productivity/titre) and explicitly...
3 claims
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Perfusion reduces bispecific antibody aggregation via mitigating mitochondrial dysfunction-induced g2020
The document investigates the relationship between upstream process parameters (comparing fed-batch vs. perfusion, varying DO, and observing differences in osmolality) and key product outcomes (VCD,...
3 claims
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Platforms, advances, and technical challenges in virus-like particles-based vaccinesFrontiers in Immunology
This review article focuses on the challenges and advances in Virus-Like Particle (VLP) production, including optimization of 'upstream and downstream processing' and monitoring 'product quality.' It...
3 claims
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Poly(Beta-Amino Ester)s as High-Yield Transfection Reagents for Recombinant Protein ProductionThe document studies the optimization of transfection reagents (PBAEs) to increase recombinant protein yield (titre/production) in CHO cell cultures. While the variable tested is the reagent...
2 claims
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Polysorbates degrading enzymes in biotherapeutics – a current status and future perspectivesThe document, a review on polysorbate-degrading enzymes (HCPs) originating from CHO cell cultures, is highly relevant to understanding challenges and process relationships in CHO systems. Although it...
3 claims
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Population balance modelling captures host cell protein dynamics in CHO cell cultures2022 · PLOS ONE
The document investigates the relationship between upstream process parameters (temperature: 36.5°C and 32°C; osmolality) and product outcomes (mAb titre, cell density/volume growth dynamics, VCD is...
3 claims
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Portable Analytical Techniques for Monitoring Volatile Organic Chemicals in Biomanufacturing Process2020 · Frontiers in Chemistry
This is a review on analytical methods (sensors, GC, MS) for monitoring upstream bioprocess variables (like pH, O2) and metabolites (VOCs). While it focuses on instrumentation rather than specific...
3 claims
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Pre-stage perfusion and ultra-high seeding cell density in CHO fed-batch culture a case study for pr2020
The document describes an experimental study using CHO cells that links upstream process parameters (pre-stage perfusion, ultra-high seeding density, lactate feeding, media composition) to product...
3 claims
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Process development of a SARS-CoV-2 nanoparticle vaccine2023
This document describes the upstream process development for a protein subunit manufactured in CHO cells. It explicitly details the monitoring of key product outcomes (VCD, viability, titer, and...
3 claims
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Process intensification for CHO cell biomanufacturing2023
The document is a PhD thesis titled 'Process intensification for CHO cell biomanufacturing.' The chapters specifically address manipulating upstream process parameters (e.g., Chapter 2: on-line...
3 claims
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Process transfer and optimization of Chinese hamster ovary cell cultivation for monoclonal antibodyThis PhD dissertation focuses on CHO cell cultivation and optimization for Monoclonal Antibody production. The abstract explicitly details experiments optimizing key upstream process parameters (DO...
3 claims
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Procollagen-lysine 2-oxoglutarate 5-dioxygenases are responsible for 5R-hydroxylysine modification o2024
The document investigates a product outcome (5R-hydroxylysine modification, a type of PTM related to glycosylation) in CHO cells and explicitly identifies relationships with upstream...
3 claims
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Production of Butyrate and Branched Chain Amino Acid Catabolic Byproducts by CHO Cells in Fed-batchThe study investigates the relationship between metabolic control (influenced by feeding parameters like glucose limitation and amino acid levels) and specific productivity (qP, a critical product...
3 claims
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Production of Functional Soluble Dectin-1 Glycoprotein Using an IRES-Linked Destabilized-Dihydrofola2012 · PLOS ONE
The document focuses on producing a recombinant glycoprotein using CHO cells and reports quantitative outcomes (titre and glycosylation) in both batch and fed-batch cultures. While the core focus is...
3 claims
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Production of Trimeric SARS-CoV-2 Spike Protein by CHO Cells for Serological COVID-19 TestingThe document directly addresses the research goal by experimentally investigating the relationship between an upstream process parameter (temperature, 37°C vs 32°C) and product outcomes (titre/yield,...
3 claims
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Production systems for recombinant antibodiesFrontiers in Bioscience
This document is a review of recombinant antibody production systems, including mammalian cells (Section 4.4), which is the host category for CHO cells. It discusses the importance of achieving high...
3 claims
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Profiling of glycosylation gene expression in CHO fed-batch cultures in response to glycosylation-enThe document investigates the relationship between upstream process parameters (feeding/medium components A and B) and product outcomes (glycosylation profile, titer, and VCD/growth) in CHO cell...
3 claims
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Proteomic Analysis of Host Cell Protein Dynamics in the Culture Supernatants of Antibody-Producing CThe document presents experimental data on CHO cell cultures comparing batch and fed-batch strategies (a type of feeding parameter). It tracks key product outcomes (Titre, VCD) and specifically...
3 claims
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Proteomic Profiling of IgG1 Producing CHO Cells Using LC LC-SPS-MS3 The Effects of Bioprocessing Con2021 · Frontiers in Bioengineering
The document directly investigates the effects of upstream process parameters (temperature, pH, dissolved oxygen concentration) on productivity (titre/production rates) and product quality (charge...
3 claims
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Proteomics Profiling of Chimeric-Truncated Tissue Plasminogen activator Producing- Chinese Hamster O2016 · Iran. Biomed.
The document describes an experimental study using CHO cells where the upstream process parameter (media composition/feeding strategy, specifically protein hydrolysate ratios and concentrations) is...
2 claims
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Proteomics Reveals Distinctive Host Cell Protein Expression Patterns in Fed‐Batch and Perfusion Cell2024
The document investigates CHO cell culture performance under different process modes (Fed-Batch vs. Perfusion) while controlling and monitoring several upstream process parameters specifically listed...
3 claims
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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automa2018 · Journal of Visualized Experiments
The document describes the analytical protocols (LC-MS for glycans, SEC-MALS for aggregation, amino acid quantification for feeding strategy) used to measure key product outcomes (glycosylation,...
2 claims
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Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automa2018 · Journal of Visualized Experiments
The document describes the analytical protocol used to measure key product outcomes (glycosylation, VCD, molecular weight/aggregation) from CHO cell cultures that were subjected to varying process...
2 claims
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QbD Implementation in Biotechnological Product Development StudiesThe document describes the Quality by Design (QbD) framework, which is the systematic approach used in biotechnological product development to identify and model multivariate relationships between...
3 claims
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Quantification of the dynamics of population heterogeneities in CHO cultures with stably integrated2020
The document investigates CHO cell population heterogeneity under different upstream conditions (high passage cultivation, varied preculture growth phases) and explicitly links these conditions to...
2 claims
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Quantitative Proteomic Analysis of Cellular Responses to a Designed Amino Acid Feed in a Monoclonal2018 · Iran. Biomed.
The document directly studies the impact of an upstream process parameter (a designed amino acid feed strategy) on a monoclonal antibody producing CHO cell line. The goal of the study is to...
3 claims
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RNASeq highlights ATF6 pathway regulators for CHO cell engineering with different impacts of ATF6β a2024
The document is an experimental study in CHO cells investigating the impact of cellular mechanisms (UPR/ATF6 pathway engineering) on key product outcomes: VCD and Titer (IgG1) during fed-batch...
3 claims
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Raman spectroscopy as a process analytical technology for pharmaceutical manufacturing and bioproces2016
The document reviews Raman spectroscopy as a Process Analytical Technology (PAT) specifically applied to bioprocessing and cell culture, explicitly mentioning Chinese Hamster Ovary (CHO) cell...
3 claims
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Rapid Identification of Chinese Hamster Ovary Cell Apoptosis and Its Potential Role in Process Robus2023
The study directly investigates the impact of upstream process parameters (temperature shift, glucose withdrawal/feeding) on CHO cell culture performance metrics (VCD, viability) and product outcomes...
3 claims
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Real-time characterization of mammalian cell culture bioprocesses by magnetic sector MSThe document describes a methodology (magnetic sector MS) for real-time tracking of critical upstream parameters (Respiratory Quotient (RQ), OUR, CER) in CHO cell cultures. These parameters are...
3 claims
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Recent Advances in Biologic Therapeutic N-Glycan Preparation Techniques and Analytical Methods for FThe document focuses on N-Glycosylation, a key product outcome (CQA), in CHO cells. While the primary topic is analytical methods (sample preparation, PAT), the introduction explicitly lists the...
2 claims
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Recent Advances in Engineering the Unfolded Protein Response in Recombinant Chinese Hamster Ovary Ce2025 · Lines. Int.
This review directly addresses the research goal by focusing on recombinant CHO cells and their product outcomes (titer, productivity, product quality/PTMs). It specifically outlines strategies for...
3 claims
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Recent Developments in Bioprocessing of Recombinant Proteins Expression Hosts and Process Developmen2019 · Frontiers in Bioengineering
This is a review article on recent developments in bioprocessing of recombinant proteins, focusing on expression hosts (including CHO cells) and process development. It explicitly mentions the...
3 claims
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Recommendation Modeling for understanding and engineering metabolism — R0 PR4The document reviews the history and principles of metabolic engineering and mathematical modeling, specifically listing 'production of protein pharmaceuticals' as an application. While not providing...
1 claim
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Recombinant therapeutic proteins degradation and overcoming strategies in CHO cells2024
The document is a mini-review focusing on Recombinant Therapeutic Protein (RTP) degradation, a key quality outcome related to titre and glycosylation, in CHO cells. It discusses factors causing...
3 claims
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Recommendation Modeling for understanding and engineering metabolism — R1 PR7The document reviews the field of metabolic modeling and engineering, specifically noting applications in the 'production of protein pharmaceuticals.' While it does not provide raw CHO experimental...
1 claim
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Removing N-terminal sequences in pre-S1 domain enhanced antibody and B-cell responses by an HBV largThe document appears to be an experimental study (likely a patent or scientific paper excerpt) detailing bioprocess conditions (labeled 61, 69, 6:) and outcomes related to the production of a protein...
2 claims
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Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media cell growth, antibody2016 · Bioresour. Bioprocess.
The document directly investigates the relationship between an upstream process parameter (feeding concentration/ratio of asparagine/glutamine) and product outcomes (cell growth/VCD and antibody...
3 claims
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Review Modeling for understanding and engineering metabolism — R0 PR2The document reviews the use of metabolic engineering and mathematical modeling for applications including the 'production of protein pharmaceuticals.' These modeling and systems biology approaches...
1 claim
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Ribosome profiling-guided depletion of an mRNA increases cell growth rate and protein secretionThe document studies an IgG-producing CHO cell line in a batch culture bioreactor system, explicitly measuring key product outcomes (Titer, VCD, specific productivity [qp]) and correlating these...
3 claims
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Robotic selection for the rapid development of stable CHO cell lines for HIV vaccine production.2018 · PLOS ONE
The document investigates two primary product outcomes (titre/expression levels and glycosylation profiles/sialic acid content) in CHO cell culture for a specific product (HIV vaccine Env). This...
3 claims
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S-Trimer, a COVID-19 subunit vaccine candidate, induces protective immunity in nonhuman primates2021
The document describes the production of a subunit vaccine (S-Trimer) using a fed-batch, serum-free culture process in CHO cells. It reports the resulting titer (~500 mg/L) and mentions 'extensive...
2 claims
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SARS-CoV-2 spike protein variant binding affinity to an angiotensin-converting enzyme 2 fusion glycoPLOS ONE
The document focuses on producing an ACE2-Fc fusion glycoprotein in CHO cell lines and explicitly characterizes a key product outcome: the N-glycan profile (glycosylation) and thermal stability....
2 claims
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SWATH-MS insights on sodium butyrate effect on mAbs production and redox homeostasis in CHO cells2024
The document directly investigates the relationship between an upstream process parameter/additive (Sodium butyrate, NaBu) and key product outcomes (titre/mAb production, VCD/growth inhibition, and...
3 claims
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Scalability of single-use biopharmaceutical manufacturing processes using process analytical technolProf. Dr.
The document investigates CHO cell culture processes, focusing on upstream parameters (feeding/medium composition, pH, DO control) and their impact on key outcomes like cumulative product titer and...
3 claims
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Scale-down bioreactors—comparative analysis of configurations2025
The document reviews scale-down bioreactors used to study the effect of gradients in upstream process parameters (pH, DO, substrate concentration/feeding) on cellular responses and Key Performance...
3 claims
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Scale-down modeling of CHO fed-batch processes for monoclonal antibody productionDipl. Biotechnol.
This doctoral thesis conducts experimental studies on CHO fed-batch processes for monoclonal antibody production. It directly investigates the relationship between a crucial upstream process...
3 claims
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Scale-down of CHO cell fed-batch cultures2018
The document is a PhD thesis focusing on 'Scale-down of CHO Cell Fed-batch Cultures.' The introduction and chapter titles indicate extensive experimental studies on CHO cells, including media...
3 claims
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Scale-up considerations for monoclonal antibody production process an oxygen transfer flux approachThe document is an experimental study on CHO cells producing a mAb. It directly investigates the relationship between upstream process parameters (specifically oxygen transfer flux (OTF), which...
1 claim
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Scale-up of CHO cell cultures from 96-well-microtiter plates to stirred tank reactors across three o2025 · Journal of Biological Engineering
The study focuses on CHO cell culture scale-up, utilizing key upstream parameters like oxygen transfer rate (OTR, related to DO) and volumetric power input (P/V, related to mixing/shear) as scale-up...
2 claims
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Scaling Fed-Batch and Perfusion Antibody Production Processes in Geometrically Dissimilar Stirred Bi2024
The document investigates upstream CHO cell culture processes (fed-batch and perfusion) for antibody production, reports key product outcomes (VCD and titre), and explicitly discusses the control of...
3 claims
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Screening Naturally Occurring Phenolic Antioxidants for Their Suitability as Additives to CHO Cell C2019
The document investigates the relationship between an upstream process parameter (media composition/addition of antioxidants, which falls under 'feeding') and key product outcomes (Viable Cell Count,...
3 claims
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Selection of chemically defined media for CHO cell fed-batch culture processes2016
This experimental study directly investigates the relationship between an upstream process parameter (feeding/media composition) and key product outcomes (titre and VCD) in CHO cell fed-batch...
3 claims
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Semi-Automated Glycoproteomic Data Analysis of LC-MS Data Using GlycopeptideGraphMS in Process DevelFrontiers in Chemistry
The document develops a semi-automated analytical method (GlycopeptideGraphMS) for characterizing and quantifying glycosylation, which is one of the key product outcomes (outputs) listed in the...
2 claims
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Sensor Selection and State Estimation of Continuous mAb Production Processes2023
The document focuses on sensor selection, state estimation (MHE), and parameter estimation within the upstream continuous mAb production process. Although it is a mathematical/control methodology...
3 claims
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Serum-Free Medium for Recombinant Protein Expression in Chinese Hamster Ovary Cells2021 · Frontiers in Bioengineering
This review focuses on CHO cells and serum-free medium components (a key upstream parameter/feeding strategy). It directly discusses the impact of optimizing these components (e.g., sugars, amino...
3 claims
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Simplifying glycan monitoring of complex antigens such as the SARS-CoV-2 spike to accelerate vaccine2023
The study investigates glycosylation (a key product outcome) in engineered CHO cell lines, which are directly relevant to the research goal. While the main focus is analytical method validation, the...
3 claims
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Simplifying the detection and monitoring of protein glycosylation during in vitro glycoengineering2023
The document details a new analytical method (GlycoSense) for the rapid, in-process monitoring of protein glycosylation (a critical product outcome listed in the goal). Although it does not report...
1 claim
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Single-Cell Analysis of CHO Cells Reveals Clonal Heterogeneity in Hyperosmolality-Induced Stress Res2022
The document directly studies the relationship between a key upstream process parameter (hyperosmolality) and CHO cell outcomes, specifically noting effects on viable cell density (VCD) and cell...
3 claims
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Site-Specific Steric Control of SARS-CoV-2 Spike GlycosylationThe document provides detailed site-specific glycosylation analysis (a key product outcome) of recombinant proteins produced in mammalian expression systems (potentially CHO, though not explicitly...
2 claims
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Soft-sensor model development for CHO growth production, intracellular metabolite, and glycan predic2024 · Front. Mol. Biosci.
The document describes developing soft-sensor models (NN, OPLS) for CHO cell cultures specifically to predict product outcomes: growth (VCD), production (titre), and glycan profiles (glycosylation)....
3 claims
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Sophisticated Cloning, Fermentation, and Purification Technologies for an Enhanced Therapeutic Prote2017 · Frontiers in Pharmacology
The document is a review focusing on sophisticated fermentation and upstream technologies for therapeutic protein production, specifically identifying CHO cells as the dominant host system. It...
3 claims
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State-of-the-Art Approaches to Heterologous Expression of Bispecific Antibodies Targeting Solid TumoThe document is a review discussing heterologous expression systems, including CHO cells, for producing complex biotherapeutics (bispecific antibodies). While the focus of the excerpt is on...
3 claims
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Strategies Used in Manufacturing Processes to Drive and Control Mammalian Cells to Undergo a Lactate2024 · Pharm. Bioprocess.
The review article directly links several upstream process parameters (pH, glucose concentration/feeding, pCO2, and subsequent osmolality changes) to critical product outcomes in mammalian/CHO cell...
3 claims
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Strategies for Fermentation Medium Optimization An In-Depth Review2017 · Frontiers in Microbiology
The document is a review of general fermentation medium optimization strategies (OFAT, RSM, ANN) and the roles of upstream parameters (pH, temperature, carbon/nitrogen sources) in maximizing...
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Streamlining the Characterization of Disulfide Bond Shuffling and Protein Degradation in IgG1 BiophaThe document focuses on characterizing disulfide bond shuffling and protein degradation in IgG1 therapeutics (rituximab and bevacizumab), which are typically CHO cell products. Although the study...
2 claims
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Study of transposon-mediated cell pool and cell line generation in CHO cells2015
The thesis provides experimental results on volumetric productivity and final titre (up to 1.5 g/L) achieved in 14-day fed-batch CHO cell cultures. This quantitative data regarding product outcome...
3 claims
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Sustained productivity in recombinant Chinese Hamster Ovary (CHO) cell lines proteome analysis of thThe document investigates product outcomes (cell specific productivity, Qp, and volumetric titre) in CHO cells during a fed-batch process which included a temperature shift (37°C to 31°C). This...
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Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glyThe document directly investigates the relationship between an upstream process parameter (glutamine concentration/feeding) and a product outcome (glycosylation) in CHO cell culture, which is the...
2 claims
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Surface plasmon resonance imaging as a screening platform in biopharmaceutical development2017
The document describes a platform (Surface Plasmon Resonance imaging) used for screening Critical Quality Attributes (CQAs) of biopharmaceuticals, specifically mentioning glycoprofiling and...
2 claims
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Systems Biology of Recombinant 2G12 and 353 11 mAb Production in CHO-K1 Cell Lines at Phosphoproteom2025
The document performs a systems biology analysis (phosphoproteomics) on CHO cells producing different monoclonal antibodies (mAb, related to product type) to understand molecular variability between...
2 claims
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Systems biology of mammalian cells mathematical modeling for uncovering metabolic compartmentationProf. Dr.
The document is a dissertation focused on using mathematical modeling and metabolic flux analysis (Inst-13CMFA) to characterize the metabolism and compartmentation of CHO-K1 cells. Understanding...
3 claims
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The Application of Dielectric Spectroscopy and Biocalorimetry for the Monitoring of Biomass in Immob2015
The document investigates methods (dielectric spectroscopy, biocalorimetry) for monitoring biomass/Viable Cell Density (VCD) in CHO cell cultures. VCD is explicitly listed as a product outcome...
3 claims
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The Challenges of Recombinant Endostatin in Clinical Application Focus on the Different Expression SThe document is a review focusing on the challenges and different expression systems (including mammalian systems) for producing recombinant Endostatin. Although the abstract favors E. coli for this...
1 claim
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The Epigenetic Regulation of Blinatumomab Gene Expression Tumor Cell-dependent T cell Response again2019
The document investigates the effect of upstream process parameters (HDAC inhibitors like sodium butyrate and valproic acid, which act as feeding/chemical additives) on product outcomes in CHO cells,...
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The Protective Effect of a Long-Acting and Multi-Target HM-3-Fc Fusion Protein in Rheumatoid Arthrit2018
The document describes the fed-batch cultivation of the HM-3-Fc fusion protein in a CHO cell line. Section 2.1 provides experimental results explicitly mentioning two of the required product...
1 claim
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The avian cell line AGE1.CR.pIX characterized by metabolic flux analysisThe document does not study CHO cells directly, but focuses on metabolic flux analysis (MFA) of an avian cell line. However, the study explicitly compares its findings to CHO metabolism, noting...
3 claims
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The effect of the chemical chaperone 4-phenylbutyrate on secretion and activity of the p.Q160R misse2019
The document investigates the effect of a chemical chaperone (4-PBA, a process/media additive) at various concentrations (5, 7.5, 10 mM) on the secretion/titre (FVII:Ag levels) of a recombinant...
3 claims
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The role of Raman spectroscopy in biopharmaceuticals from development to manufacturing2021
This review discusses the role of Raman spectroscopy as a Process Analytical Technology (PAT) in bioprocessing, specifically focusing on monitoring and controlling upstream parameters (like pH,...
3 claims
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Therapeutic Fc fusion protein misfolding A three-phasic cultivation experimental design2019 · PLOS ONE
The document directly studies the relationship between an upstream process parameter (temperature shift and timing) and key product outcomes (protein titer and protein folding/quality) in CHO cell...
3 claims
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Therapeutic proteins facing the challenges of glycobiologyThe document is a review focusing on the relationship between critical production parameters (culture conditions) and glycosylation (a key product outcome) in therapeutic protein manufacturing,...
3 claims
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Thrombomodulin is essential for recombinant Fc-fusion protein production in Chinese hamster ovary ce2025 · Journal of Biological Engineering
The document investigates the relationship between an internal cell parameter (Thrombomodulin gene expression) and product outcomes (titre/yield and VCD/viability) in CHO cell culture. While the...
3 claims
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Towards Autonomous Process Control—Digital Twin for CHO Cell-Based Antibody Manufacturing Using a Dy2022
The document investigates a dynamic metabolic model for CHO cell antibody manufacturing and uses statistical evaluation to identify significant influencing factors (nutrient concentrations/feeding...
3 claims
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Towards Enhancing Therapeutic Glycoprotein Bioproduction Interventions in the PI3K AKT mTOR Pathway2019
The document focuses on CHO cells, titre (productivity), VCD, and glycosylation (PTMs), which are the target outputs and host system of the research goal. While the primary intervention discussed is...
3 claims
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Transcriptomic profiling as a tool to characterize CHO cell processes2018
The thesis focuses on characterizing CHO cell processes under different upstream process conditions (scale-up/down, fed-batch, perfusion) using transcriptomics. Chapter 1 explicitly mentions the need...
3 claims
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Transient gene expression for rapid protein production studies & optimizations under serum-free2005
This document is a PhD thesis focused on optimizing transient gene expression (TGE) systems for large-scale protein production using CHO DG44 cells under serum-free conditions. The summary explicitly...
3 claims
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Transient recombinant protein expression in mammalian cells the role of mRNA level and stability2009
The document investigates the effect of an upstream process parameter, specifically hypothermia (temperature variation), on product outcome (recombinant antibody yield/titre) in CHO DG44 cell...
3 claims
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Trends in Upstream and Downstream Process Development for Antibody Manufacturing2014
This review paper covers trends and optimization strategies in upstream processing (USP) for antibody manufacturing, primarily using CHO cells. It explicitly discusses the optimization of process...
3 claims
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Trivalent soluble TNF Receptor, a potent TNF-α antagonist for the treatment collagen-induced arthrit2018
The document focuses on producing a biological drug (TNFRII-Trimer) using CHO cells. It specifically mentions screening for high-titer production clones and utilizing fed-batch cell culture. Although...
2 claims
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Understanding and Controlling Sialylation in a CHO Fc-Fusion Process2016 · PLOS ONE
The study directly investigates the relationship between an upstream process parameter (Dissolved Oxygen/oxidative stress) and a product quality outcome (sialylation, a form of glycosylation) in a...
3 claims
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Understanding the impact of different bioprocess conditions on monoclonal antibody glycosylation inThe document directly investigates the relationship between an upstream process parameter (temperature/mild hypothermia) and product outcomes (mAb glycosylation and specific productivity/titre) in...
3 claims
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Unravelling Immunoglobulin G Fc N-Glycosylation A Dynamic Marker Potentiating Predictive, Preventive2018
The document is a review focused entirely on factors influencing Immunoglobulin G (IgG) N-glycosylation, which is a critical product outcome mentioned in the research goal. Although the context is...
1 claim
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Uridine modulates monoclonal antibody charge heterogeneity in Chinese hamster ovary cell fed-batch c2018 · Bioresour. Bioprocess.
The document investigates the relationship between an upstream parameter (Uridine/Feeding) and multiple product outcomes (VCD, Titre, and Glycosylation/PTMs like charge heterogeneity) in CHO cell...
3 claims
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Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells2018 · Journal of Visualized Experiments
The document describes a methodology using CHO cells in a high-throughput system (microbioreactor/ambr) that is explicitly designed to conduct Design of Experiments (DOE) linking upstream process...
1 claim
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Use of S-sulfocysteine (SSC) as a L-cysteine source in Chinese hamster ovary (CHO) suspension batchJournal of Biotechnology
The document investigates the use of S-sulfocysteine (a feeding parameter/upstream process parameter) in CHO cell cultures (matching the system). The methods section (Table of Contents, Sections 4.4,...
3 claims
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Using Molecular Markers to Characterize Productivity in Chinese Hamster Ovary Cell Lines2013 · PLOS ONE
The document investigates CHO cell culture productivity (qp) and VCD (biomass, growth rate) and their variation among different cell lines, which are key output and comparison parameters for the...
3 claims
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Using simple models to describe the kinetics of growth, glucose consumption, and monoclonal antibody2015
The document directly studies the kinetics of CHO cell growth (VCD) and monoclonal antibody formation (Titre) based on upstream parameters, specifically varying temperature (33°C vs 37°C) and initial...
3 claims
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Utilizing non-clonal CHO cell derived materials for preclinical studies of complex molecules2025
The document investigates CHO cell culture performance, productivity (titer), and product quality (including N-glycan structures/glycosylation) for different cell line sources (clonal vs. non-clonal...
3 claims
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Virus-like particles as a highly efficient vaccine platform Diversity of targets and production syst2012
The document is a review covering Virus-Like Particle (VLP) production systems, including 'Mammalian and avian cells' (Section 3.4). Although it is a high-level review and is unlikely to contain...
1 claim
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ambr™ Mini-bioreactor as a high-throughput tool for culture process development to accelerate transfThe document describes an experimental study using CHO cells producing a monoclonal antibody. It monitors and compares all required upstream process parameters (temperature, pH, DO, osmolality,...
2 claims
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mAb production kinetics in CHO batch culture exploring extracellular and intracellular dynamicsThe document focuses on mAb production kinetics (product outcome/titre) in CHO cells and explores the dynamics of key extracellular and intracellular substrates and metabolites (feeding/nutrient...
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| Document | Claims |
|---|---|
Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems Natalie Muller Æ Madiha Derouazi Æ Fre´de´ric Van Tilborgh Æ Sarah Wulhfard Æ2006 · Cole Polytechnique F D Rale De Lausanne Epfl
The paper describes CHO cell culture experiments (transient expression) over various scales, explicitly reporting the control of several key upstream parameters (pH and DO maintained,...
3 claims
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Reduced glutamine concentration improves protein production in growth-arrested CHO-DG44 and HEK-293E cells Yashas Rajendra • Divor Kiseljak • Lucia Baldi •2011 · Cole Polytechnique F D Rale De Lausanne Epfl
The study investigates the effect of varying glutamine concentration (an upstream feeding/media parameter) on recombinant protein yield (titre) in CHO-DG44 cells. It also measures cell density and...
3 claims
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Trimeric SARS-CoV-2 Spike Proteins Produced from CHO Cells in Bioreactors Are High-Quality Antigens2020 · Cole Polytechnique F D Rale De Lausanne Epfl
The document describes a scalable process for producing a recombinant protein (SARS-CoV-2 Spike) using CHO cells in bioreactors. The methods section explicitly mentions the evaluation of 'cell...
2 claims
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de nationalité suisse et originaire de Eggiwil ( BE ) acceptée sur proposition du jury2007 · Cole Polytechnique F D Rale De Lausanne Epfl
The document is a PhD thesis concerning 'bioreactor processes for the production of recombinant proteins from mammalian cells.' This field inherently involves studying the impact of upstream...
3 claims
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acceptée sur proposition du jury: Prof. F. Radtke, président du jury Prof. F. M. Wurm, Dr M. Jordan, directeurs de thèse Prof. J. Auwerx, rapporteur2012 · Cole Polytechnique F D Rale De Lausanne Epfl
The document is a PhD thesis focusing specifically on CHO cell culture metabolism (lactate production/consumption, glutamine depletion, mitochondrial function). Understanding these metabolic shifts...
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Dipl.-Ing. Biotechnologie, Technische Universität Berlin, Allemagne et de nationalité allemande acceptée sur proposition du jury: Prof U. von Stockar, directeur de thèse2002 · Cole Polytechnique F D Rale De Lausanne Epfl
The thesis directly investigates the relationship between upstream process parameters (specifically temperature and nutrient availability/precursor feeding) and a specified product outcome...
3 claims
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Optimization of the medium perfusion rate in a packed-bed bioreactor charged with CHO cells F. Meuwly1, U. von Stockar2,* & A. Kadouri12004 · Cole Polytechnique F D Rale De Lausanne Epfl
The document describes an experimental study in CHO cell culture focused on optimizing an upstream process parameter (medium perfusion rate, which relates to feeding/dilution) and documenting the...
3 claims
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Institut des sciences et ingénierie chimiques2005 · Cole Polytechnique F D Rale De Lausanne Epfl
The thesis focuses on optimizing transient gene expression and large-scale protein production in CHO DG44 cells under serum-free conditions. The summary explicitly mentions achieving high cell...
3 claims
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Recombinant therapeutic proteins were introduced >20 years ago and now generate >$99 billion in annual revenue from a broad range of2011 · Hong Kong University Of Science And Technology Hkust
The document provides the draft genome sequence of the CHO-K1 cell line and specifically investigates genes involved in glycosylation (a key product outcome listed in the research goal) and their...
2 claims
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Continuous Online Protein Quality Monitoring during Perfusion Culture Production Using an Integrated Micro/Nanofluidic System The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters2020 · Massachusetts Institute Of Technology Mit
The document presents a novel micro/nanofluidic system for continuous, online monitoring of product quantity (titre) and quality (size distribution) during CHO perfusion culture. Although it is a...
3 claims
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Dissecting N-Glycosylation Dynamics in Chinese Hamster Ovary2019 · Massachusetts Institute Of Technology Mit
The document directly studies N-glycosylation dynamics (product outcome) in CHO cells cultivated using two distinct fed-batch processes. It measures viable cell density (VCD), titre, and...
3 claims
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Investigating the influence of physiologically relevant hydrostatic pressure on CHO cell batch culture The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters2021 · Massachusetts Institute Of Technology Mit
The study directly investigates the relationship between a physical upstream process parameter (hydrostatic pressure) and key product outcomes (maximum cell concentration/VCD and IgG1 titre) in a CHO...
3 claims
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operated at the physiological temperature of 37oC. Low temperature cell culture at 30-33oC, in particular for CHO cells, increased the specific productivity of many recombinant proteins amongstMassachusetts Institute Of Technology Mit
The document directly studies the relationship between an upstream process parameter (temperature) and product outcomes (VCD/growth, specific productivity, total yield) in CHO cell cultures. The...
3 claims
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Glycosylation Site Occupancy Heterogeneity in Chinese Hamster Ovary Cell Culture1998 · Massachusetts Institute Of Technology Mit
This Ph.D. thesis directly studies the relationship between upstream process parameters (continuous culture/chemostat, batch/fed-batch, nutrient limitation, media feeding strategies) and a key...
3 claims
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Active hypothermic growth: a novel means for increasing total recombinant protein production by CHO cells2005 · Massachusetts Institute Of Technology Mit
The document, a PhD thesis abstract on CHO cell culture, directly investigates the relationship between an upstream process parameter (temperature/hypothermia) and product outcomes (recombinant...
3 claims
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The Effect of Culture Temperature on Recombinant IFN-γ Production and QualityMassachusetts Institute Of Technology Mit
The document directly studies the effect of a key upstream process parameter (culture temperature) on multiple product outcomes (titre/production and glycosylation quality) in CHO cell cultures,...
3 claims
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Reduced Temperature Production of Recombinant Proteins to Increase Productivity in Mammalian Cell Culture Steve R. Fox1, Daniel I.C. Wang2, Miranda Yap3Massachusetts Institute Of Technology Mit
Classification failed: no response from batch API
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Increasing IFN-y Productivity in CHO Cells through CDK Inhibition David Alan McClain B.S. Chemical Engineering Cornell University, Ithaca, NY, 20042010 · Massachusetts Institute Of Technology Mit
The document is a doctoral thesis focused entirely on increasing productivity (titre) in CHO cells. It directly investigates the relationship between cellular state (growth arrest induced by CDK...
3 claims
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Ovary Cells for Low-temperature Culture TAN Hong Kiata, Miranda Yapb, Daniel I.C.Wangc a Singapore-MIT Alliance, 4 Engineering Drive 3, National University of Singapore, Singapore-Massachusetts Institute Of Technology Mit
The document investigates the relationship between an upstream process parameter (low temperature, 30-33°C) and cell characteristics (increased specific productivity and extended viability) in CHO...
1 claim
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improve quality of recombinant glycoproteins involve theMassachusetts Institute Of Technology Mit
The document investigates product outcome (sialylation/glycosylation) in CHO cells. Although the main experiment uses genetic engineering, the introduction extensively reviews and documents the...
3 claims
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Enhancing CHO cell productivity through a dual selection system using Aspg and Gs in glutamine free mediumTechnical University Of Denmark Dtu
The document investigates CHO cell culture performance (titre, specific productivity, VCD/growth rate) as product outcomes, correlating them with changes in the cellular system and selection media...
3 claims
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UC Davis Electronic Theses and DissertationsUniversity Of California Davis
The document is a thesis (relevant source type) that addresses the research goal's key components. Chapters 5-7 detail atomistic modeling studies focusing on the interaction and binding of...
3 claims
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UC Davis Electronic Theses and Dissertations2021 · University Of California Davis
The document is a dissertation focused on CHO cells and bioproduction. The abstract explicitly mentions maximizing titers, investigating the impact of engineered systems on 'protein quality' (which...
3 claims
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Enhancing the functionality of a microscale bioreactor system as an industrial process development tool for mammalian perfusion culture David J Sewell1 | Richard Turner2 | Ray Field2 | William Holmes2 | Rahul Pradhan2 |2018 · University Of Cambridge
The document describes an experimental study using CHO cells in perfusion culture, investigating the effect of process parameters (pH, DO concentrations, mixing) on key product outcomes, including...
3 claims
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The Biological Impact of High Throughput Continuous Bioprocessing David John Sewell Churchill College Thesis submitted for the degree of Doctor of Philosophy2020 · University Of Cambridge
The document is a PhD thesis focusing on developing continuous bioprocessing (perfusion/fed-batch) for CHO cell cultures. The abstract specifically discusses the impact of upstream parameters (pH and...
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The Assessment of MAb Quality and Quantity2015 · University Of Guelph
The document is an experimental thesis focused on CHO cell culture optimization. It directly investigates the relationship between upstream process parameters (temperature shift, media...
3 claims
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C e l l C u l t u r e a n d T i s s u e E n g i n e e r i n g Effect of adenosine and cordycepin on recombinant antibody production yields in two different Chinease hamster ovary cellUtrecht University
The document investigates the effect of upstream chemical supplements (adenosine, cordycepin) and a key process parameter (glucose feeding) on product outcomes (antibody titer, productivity) and cell...
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Glycobiology vol. 10 no. 1 pp. 77–87, 2000Utrecht University
The document investigates product outcomes (protein folding, secretion, and glycosylation structure—Man5 N-glycans) in CHO cells. It explicitly links the upstream process parameter 'temperature' to...
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| Document | Claims |
|---|---|
US-10208299-B22019
The document focuses on achieving a highly specific and optimized glycosylation profile (M6P content, a key product outcome) for a therapeutic protein (rhGAA) produced in CHO cells. Although the...
3 claims
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US-10377833-B22019
The document describes a specific CHO mutant cell line (Slc35C1 dysfunctional) engineered to produce antibodies with a modified glycosylation profile (afucosylation). Although the primary focus is...
3 claims
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US-10435464-B12019
The patent claims detail methods for producing a therapeutic protein (Adalimumab) using CHO cells, specifying control over key upstream parameters, particularly the 'feeding' strategy (initial and...
3 claims
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US-10611831-B22020
The document focuses on CHO cell line engineering leading to improved output parameters (Titre, VCD) for specific product types (antibodies). Although it does not directly study the correlation...
3 claims
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US-11014981-B22021
The document is a patent describing a method for producing a recombinant monoclonal antibody using CHO cells. Claim 1 explicitly links specific ranges for upstream process parameters (pH,...
3 claims
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US-11396540-B12022
The document details methods for CHO cell culture producing antibodies (adalimumab). It explicitly studies the manipulation of an upstream parameter (glucose concentration and alternate hexose...
2 claims
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US-11505609-B22022
The document is highly relevant to the domain (CHO cell culture, glycosylation/product outcome), and discusses variability based on cell line type (FUT8 knockout). Although the primary focus is on...
3 claims
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US-11505784-B22022
The document, a patent on CHO cell culture media, focuses on establishing the relationship between upstream process parameters (feeding composition, specifically novel amino acid ratios and iron...
3 claims
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US-11591583-B22023
The document describes a CHO cell derived product (rhGAA) with a specifically optimized glycosylation profile (high M6P), which is a key product outcome of interest. While the visible abstract and...
3 claims
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US-12246062-B22025
The document describes a recombinant protein (rhGAA) expressed in CHO cells, focusing heavily on a critical product outcome (glycosylation, specifically M6P and sialic acid content). Although the...
3 claims
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US-12351641-B22025
The document is a patent focused on CHO cell culture processes. It explicitly studies the relationship between an upstream parameter (media composition/feeding, specifically glutamine-free medium...
3 claims
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US-2002019031-A12002
The document, a patent on CHO cell culture methods, establishes a direct relationship between the cell cycle phase (G1 arrest/minimum specific growth rate) and the product formation rate/productivity...
3 claims
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US-2003035799-A12003
The document focuses on CHO cell lines producing antibodies, specifically mentioning the product outcome 'CHO glycosylation.' It also addresses upstream process parameters by defining medium...
3 claims
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US-2003148493-A12003
The document focuses on engineering a CHO cell line to disrupt sialidase expression, which directly relates to controlling the product outcome of **glycosylation** (specifically sialylation) in CHO...
3 claims
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US-2003190710-A12003
The document is a patent describing methods to control a product quality attribute (level of non-glycosylated heavy chain, which relates to glycosylation) in CHO cell culture by adjusting upstream...
3 claims
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US-2004214289-A12004
The document is a patent describing an experimental method in CHO cell culture that correlates upstream process parameters (temperature and osmolality) with a specific product outcome...
3 claims
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US-2006115901-A12006
The document focuses on optimizing specific media components (L-glutamine, iron, insulin, growth factors, antioxidants) for CHO cell growth and survival (which relates to VCD, a key outcome). This...
2 claims
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US-2010021911-A12010
The document focuses on developing specific CHO host cell lines (DSM ACC2909, DSM ACC2910) and selecting them based on a high Integral of Viable Cells (IVC), which is closely related to VCD, a key...
3 claims
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US-2010132059-A12010
The document investigates the relationship between an upstream process parameter (Temperature, specifically below 37°C) and gene expression in CHO cells. This provides direct experimental findings on...
2 claims
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US-2011111495-A12011
The document is a patent describing a concentrated medium formulation for large-scale fed-batch culture of CHO cells. The composition and implementation of concentrated feed media (feeding...
3 claims
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US-2011177555-A12011
The document focuses on improving a key product outcome (sialylation/glycosylation) in CHO cells through cell line engineering (GnT I modification), and references adapting the cell line to...
3 claims
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US-2011275121-A12011
The document focuses on enhancing protein production (titre/secretion) in CHO cell cultures through genetic modification (HBx/XBP1s to activate UPR), thereby addressing the 'product outcomes' aspect...
3 claims
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US-2013029413-A12013
The document focuses on enhancing a key product outcome, glycosylation (sialylation), specifically mentioning CHO cells (Claim 30). While the methods involve metabolic engineering rather than...
3 claims
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US-2013129755-A12013
The document defines a specific genetically modified CHO cell line (GnT I deficient, JW152) designed to control the output of glycosylation (mannose-terminated N-glycans), which is a key product...
2 claims
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US-2013130316-A12013
The document is a patent describing an optimized CHO cell culture process. It explicitly documents the relationship between upstream process parameters (temperature shifts, pH shifts, and specific...
2 claims
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US-2014011237-A12014
The document provides experimental data comparing two CHO cell lines (CAT-S vs. CHO-S) under fed-batch culture conditions, focusing on the product outcome (recombinant IgG titre) over time (Day 14)....
2 claims
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US-2014057316-A12014
The document describes a method for producing a polypeptide in CHO cells. Claim 8 explicitly details the use of an upstream process parameter—an incubation temperature shift (37°C to 32°C)—to...
2 claims
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US-2014335609-A12014
The patent focuses primarily on cell line engineering (transfection and selection methods) rather than systematically documenting relationships between process parameters and outcomes. However, it...
3 claims
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US-2015044719-A12015
The document is a patent focused on CHO cell culture methods using specific chemically defined media compositions (e.g., high glucose concentration, specific supplements like putrescine and...
3 claims
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US-2015184135-A12015
The document focuses on engineering a CHO cell line to achieve higher sialylation (a form of glycosylation), which is a key product outcome mentioned in the research goal. While it addresses cell...
3 claims
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US-2015299644-A12015
The document details a novel upstream bioprocess methodology (discontinuous/cyclic fed-batch with cell recycle) specifically for CHO cells producing biological substances (antibodies). While it does...
3 claims
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US-2016185847-A12016
The document focuses on monoclonal antibodies produced in CHO cell lines and defines specific desired product outcomes related to glycosylation (fucose and galactosylation levels). While the provided...
2 claims
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US-2016194369-A12016
The document describes methods using CHO cells to increase product outcomes (titre and cell growth/VCD) by manipulating the IL6 signaling pathway. While the input parameter (IL6...
2 claims
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US-2017073399-A12017
The document focuses on producing a specific recombinant protein (eculizumab) in CHO cells and achieving desired product outcomes, specifically detailed glycosylation patterns (NGNA, GalNAc, neutral...
2 claims
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US-2018022820-A12018
The document describes a Chinese Hamster Ovary (CHO) mutant cell line engineered to have a dysfunctional Slc35C1 gene, specifically resulting in reduced fucosylation (a form of glycosylation). This...
2 claims
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US-2018346881-A12018
The document, a patent, focuses on optimizing upstream process parameters (media composition/feeding, specifically amino acid ratios and iron carriers) in CHO cell culture to achieve improved product...
3 claims
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US-2019055513-A12019
The document focuses on using a modified upstream process parameter (substituting traditional carbohydrates with maltose in the media/feeding) specifically in CHO cell cultures. The claims and...
3 claims
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US-2019225694-A12019
The document focuses on CHO DG44 cell cultures and heavily details a specific product outcome: the N-glycan content (glycosylation profile) of the expressed biosimilar antibody. The claims and...
3 claims
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US-2019270800-A12019
The document focuses on enhancing CHO cell production (titre, VCD) by modifying the cell line (EDEM2/XBP1 expression). While it does not explicitly study the relationship between physical process...
3 claims
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US-2019276812-A12019
The document defines specific, highly optimized glycosylation parameters (M6P residues, complex type N-glycans) for a product derived from CHO cells. While the provided abstract and claims focus on...
3 claims
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US-2020123244-A12020
The document is a patent describing the production of a monoclonal antibody in CHO cells. Claims 11, 12, 13, and 14 explicitly define optimized upstream process parameters including pH, temperature,...
3 claims
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US-2021155670-A12021
The document utilizes CHO cells to produce a specific glycosylated protein product (MHC-I complexes). While the excerpt focuses on the product structure (claims) rather than upstream process...
3 claims
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US-2021317435-A12021
The document focuses on engineering a CHO cell line (DHFR−/− GS−/−) for industrial production of therapeutic proteins and selecting clones based on increased titre (product outcome). It explicitly...
1 claim
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US-2021214702-A12021
This patent describes a CHO cell culture process for recombinant TNK-tPA production, specifically optimizing several key upstream parameters (temperature, pH, DO level, osmolality) and documenting...
3 claims
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US-2022154137-A12022
The document, a patent, focuses on developing new culture media compositions (maltose and other saccharides) for CHO cells to increase protein yield (titre) and support growth (VCD). Modifying the...
3 claims
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US-2022306979-A12022
The document focuses on developing data-driven models (soft sensors) to determine process-relevant parameters in CHO cell cultures (Claim 13, 14). It explicitly uses upstream process variables...
3 claims
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US-2022324952-A12022
The document describes the production of a recombinant protein (Eculizumab) in CHO cells, focusing heavily on achieving specific glycosylation patterns (a required output parameter). The claims...
2 claims
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US-2022220509-A12022
The document reports experimental data on key product outcomes (titre/productivity and viability) in CHO cells cultivated under fed-batch conditions. While the primary independent variable studied is...
2 claims
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US-2022348977-A12022
The document is a patent describing CHO cell culture methods. It explicitly manipulates two key upstream process parameters: temperature (temperature shift from 37°C to 30°C) and feeding/nutrient...
3 claims
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US-2023203449-A12023
The document focuses on manipulating upstream process parameters (basal and feed medium composition, specifically novel amino acid ratios and iron carriers) in CHO cell culture processes to achieve...
3 claims
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US-2023203169-A12023
The document directly addresses CHO cells and a critical product outcome (glycosylation profile control), and how this outcome is achieved using a specific cell line variation (FUT8 knockout CHO)....
3 claims
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US-2024066104-A12024
The document is a patent describing a recombinant protein expressed in CHO cells and specifies a critical product outcome (increased mannose-6-phosphate glycosylation). Although the provided abstract...
3 claims
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US-2024083972-A12024
The document describes methods for optimizing the N-1 CHO cell culture stage using specific flow rates, viscosity, and density parameters (upstream process parameters) to control cell aggregation....
3 claims
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US-2024158754-A12024
The document, a patent focusing on CHO cell culture, details a specific method involving the manipulation of a process parameter (adding GMP/related agents, which falls under 'feeding/media...
3 claims
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US-2024190944-A12024
The document focuses on improving product outcomes (titre, viability) in CHO cells, which aligns with the desired outputs and cell system. While the inputs studied are genetic modifications (BAX,...
3 claims
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US-4808523-A1989
The document describes a method for producing IFN-beta 1 using CHO cells, a relevant cell line and product type (glycoprotein). It specifies key upstream parameters (e.g., operating temperature of...
3 claims
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US-5122469-A1992
The document is a patent describing specific culture media formulations (upstream process parameters: feeding, osmolality defined at 350 mOsm) for CHO cells designed to 'improve production of...
3 claims
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US-5053334-A1991
The document details a method for operating recombinant CHO cell suspension cultures (specifically using medium exchange via cross-flow filtration to enable high-density culture) to produce t-PA....
2 claims
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US-5221620-A1993
The document is an experimental patent describing high-level expression of TGF-beta 2 in CHO cells. It explicitly documents product outcomes, including titre ('approximately 5 μg per ml culture...
3 claims
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US-5272070-A1993
The document focuses entirely on creating a specialized CHO cell line to control and homogenize N-linked glycosylation (a key product outcome). Although it does not document the relationship between...
3 claims
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US-5316938-A1994
The document defines a culture medium for engineered CHO cells and explicitly sets target ranges for key upstream parameters (osmolality and pH) necessary for supporting cell growth and product...
2 claims
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US-5510261-A1996
The document focuses on CHO cells and their production of glycoproteins. The core mechanism described is controlling a product outcome (glycosylation, specifically sialic acid content) by...
2 claims
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US-5545404-A1996
The document focuses on CHO cell lines for antibody production and specifically mentions 'antibody having CHO glycosylation,' which aligns with the cell line and product outcome criteria...
3 claims
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US-5871999-A1999
The document investigates the manipulation of upstream process parameters (molar ratio of inorganic ions to amino acids) in CHO cell culture. Changes in ion concentration directly affect osmolality,...
3 claims
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US-6069235-A2000
The document describes a method using CHO cells where an upstream chemical input (N-butyl deoxynojirimycin concentration, 0.5-2 mM) is controlled during cell culture maintenance conditions, and this...
3 claims
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US-6458565-B12002
The document details experimental methods for CHO cell culture optimization (upstream process). It specifically investigates the relationship between the feeding strategy (type of medium and...
3 claims
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US-6562588-B22003
The document focuses on engineering CHO cell lines (disrupting sialidase expression) specifically to improve a key product outcome, glycosylation (sialylation), which is a listed output in the...
3 claims
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US-6924124-B12005
The document is a patent describing experimental methods in CHO cell culture. It specifically details the relationship between an upstream process parameter (feeding/nutrient concentration,...
3 claims
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US-8765413-B22014
This patent describes an experimental CHO cell culture process based on optimizing upstream process parameters (temperature shifts and pH shifts) to improve product outcomes (increased yield/titre,...
3 claims
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US-8846373-B22014
The document explicitly addresses methods to promote sialylation (a type of glycosylation, which is a required product outcome) and specifically names CHO cells (Claim 28) as a host cell line. While...
3 claims
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US-9012176-B22015
The document focuses on developing a mutant CHO cell line specifically capable of achieving higher protein sialylation (a key form of glycosylation), which is one of the target product outcomes...
3 claims
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US-9068198-B22015
The document, a patent describing a new CHO cell line (CAT-S), provides experimental data comparing its performance (recombinant IgG titre, a product outcome) against a standard CHO-S line. This...
3 claims
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US-9079954-B22015
The document focuses on enhancing CHO cell productivity through genetic engineering (EDEM2/XBP1 expression), which results in high cell line specific productivity. It reports key outcomes relevant to...
3 claims
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US-9222081-B22015
The document describes a method for producing a polypeptide in CHO cells (Chinese Hamster Ovary cells). Claim 8 specifically details the use of an upstream process parameter control, mentioning an...
2 claims
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US-9243235-B22016
The document focuses specifically on CHO cells and a critical product outcome: glycosylation (mannose-terminated N-glycans). While the mechanism described is cell line engineering (GnT I mutation)...
3 claims
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US-9273292-B22016
The document focuses on Chinese Hamster Ovary (CHO) cells and directly addresses one of the key product outcomes of the research goal: glycosylation (specifically, increased sialylation). Although it...
3 claims
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US-9340592-B22016
The document is an experimental study in CHO cell culture reporting how cell line variation (engineering to increase CERT S132A expression) affects product outcomes (specific productivity/pcd). This...
3 claims
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US-9340814-B22016
The document focuses on optimizing culture methods for CHO cells, specifically altering upstream parameters related to feeding and medium composition (e.g., high glucose concentration,...
3 claims
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US-9382315-B22016
The document focuses on improving key product outcomes (titre) and growth metrics (integrated cell density/VCD) in CHO cells through genetic modification (EDEM2 expression). While it does not...
3 claims
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US-9982226-B22018
The document details a high-efficiency, discontinuous fed-batch culture method for CHO cells involving cell recycle and alternating bioreactors. While the focus is on system configuration rather than...
3 claims
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US-RE39792-E2007
The patent defines specific, quantifiable operating ranges for several key upstream process parameters (osmolality, pH, and detailed feeding components/concentrations) necessary for growing CHO...
2 claims
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US-RE48651-E2021
The document investigates CHO cell lines and reports improved product outcomes (titre and VCD), directly addressing the 'product outcomes' and 'vary by cell line and product type' aspects of the...
3 claims
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